Molecular cloning and characterization of low-molecular-weight glutenin sequences from an old Hungarian wheat variety, Bánkúti 1201

I. J. Nagy, Imre Takács, L. Tamás, Z. Bedő

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The old Hungarian wheat variety Bánkúti 1201 was widely grown in Hungary mainly because of its outstanding breadmaking quality. In order to isolate and preserve the genes of this remarkable variety a cDNA library was created from the immature kernels of a selected line of Bánkúti 1201.When the library was screened a large number of LMW glutenin-specific clones were isolated and of these eight genes were finally sequenced. The sequence alignments revealed several new LMW glutenin genes. The results obtained so far suggest that this old variety may carry many other unknown storage protein genes of either theoretical or commercial importance.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalCereal Research Communications
Volume31
Issue number1-2
Publication statusPublished - 2003

Fingerprint

glutenins
Molecular Cloning
Triticum
molecular cloning
Molecular Weight
molecular weight
wheat
Genes
genes
Hungary
Sequence Alignment
breadmaking quality
Gene Library
Libraries
sequence alignment
storage proteins
Clone Cells
cDNA libraries
preserves
immatures

Keywords

  • CDNA
  • Cloning
  • Glutenin
  • Sequencing
  • Wheat

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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T1 - Molecular cloning and characterization of low-molecular-weight glutenin sequences from an old Hungarian wheat variety, Bánkúti 1201

AU - Nagy, I. J.

AU - Takács, Imre

AU - Tamás, L.

AU - Bedő, Z.

PY - 2003

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AB - The old Hungarian wheat variety Bánkúti 1201 was widely grown in Hungary mainly because of its outstanding breadmaking quality. In order to isolate and preserve the genes of this remarkable variety a cDNA library was created from the immature kernels of a selected line of Bánkúti 1201.When the library was screened a large number of LMW glutenin-specific clones were isolated and of these eight genes were finally sequenced. The sequence alignments revealed several new LMW glutenin genes. The results obtained so far suggest that this old variety may carry many other unknown storage protein genes of either theoretical or commercial importance.

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KW - Cloning

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KW - Sequencing

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