Molecular characterization of two different strains of haemotropic mycoplasmas from a sheep flock with fatal haemolytic anaemia and concomitant Anaplasma ovis infection

S. Hornok, Marina L. Meli, András Erdos, István Hajtós, Hans Lutz, Regina Hofmann-Lehmann

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

After the first outbreak of fatal Mycoplasma ovis infection (eperythrozoonosis) in a sheep flock in Hungary (1997), a second wave of the disease was noted in 2006, with different seasonal pattern and affected age group, as well as increased mortality (5.5%). The aim of the present study was to molecularly characterize the causative agent and to reveal underlying factors of the second wave of the disease. Remarkably, among the 33 sheep examined, 17 were infected with two strains of haemotropic mycoplasmas. Cloning and sequencing isolates of the latter showed that one of the strains was 99.4-99.8% identical to M. ovis (AF338268), while the second was only 96.8-97.9% identical and contained a 17-bp deletion. Different isolates of both strains were demonstrated in the same animal. When analyzing possible risk factors for fatal disease outcome, we found that among sheep born prior to the 1997 outbreak significantly more animals survived the second outbreak than succumbed to disease. In addition, locally born sheep were less frequently diseased than sheep introduced into the flock from other places. This suggests an immunoprotective effect in some animals. Concurrent infection with Anaplasma ovis was detected in 24 of the 33 evaluated sheep. In conclusion, this is the first study to demonstrate the existence of and characterize two genetically distinct ovine haemotropic mycoplasma strains in a sheep flock with fatal haemolytic anaemia.

Original languageEnglish
Pages (from-to)372-377
Number of pages6
JournalVeterinary Microbiology
Volume136
Issue number3-4
DOIs
Publication statusPublished - May 12 2009

Fingerprint

Anaplasma ovis
hemolytic anemia
Mycoplasma
Hemolytic Anemia
Sheep
flocks
sheep
Infection
infection
Mycoplasma ovis
Mycoplasma Infections
Disease Outbreaks
Sheep Diseases
mycoplasmosis
animals
Fatal Outcome
Hungary
mixed infection
risk factors
Organism Cloning

Keywords

  • Anaemia
  • Anaplasma ovis
  • Haemotropic mycoplasmas
  • Mycoplasma ovis
  • Sheep

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Molecular characterization of two different strains of haemotropic mycoplasmas from a sheep flock with fatal haemolytic anaemia and concomitant Anaplasma ovis infection. / Hornok, S.; Meli, Marina L.; Erdos, András; Hajtós, István; Lutz, Hans; Hofmann-Lehmann, Regina.

In: Veterinary Microbiology, Vol. 136, No. 3-4, 12.05.2009, p. 372-377.

Research output: Contribution to journalArticle

Hornok, S. ; Meli, Marina L. ; Erdos, András ; Hajtós, István ; Lutz, Hans ; Hofmann-Lehmann, Regina. / Molecular characterization of two different strains of haemotropic mycoplasmas from a sheep flock with fatal haemolytic anaemia and concomitant Anaplasma ovis infection. In: Veterinary Microbiology. 2009 ; Vol. 136, No. 3-4. pp. 372-377.
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