Molecular characterization of the Rpt1/p48B ATPase subunit of the Drosophila melanogaster 26S proteasome

Áron Szabó, Margit Pál, Péter Deák, Petra Kiss, Zsuzsa Újfaludi, Tibor Pankotai, Zoltán Lipinszki, Andor Udvardy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The function and the molecular properties of the Rpt1/p48B ATPase subunit of the regulatory particle of the Drosophila melanogaster 26S proteasome have been studied by analyzing three mutant Drosophila stocks in which P-element insertions occurred in the 5′-non-translated region of the Rpt1/p48B gene. These P-element insertions resulted in larval lethality during the second instar larval phase. Since the Rpt1/p48B gene resides within a long intron of an annotated, but uncharacterized Drosophila gene (CG17985), the second instar larval lethality may be a consequence of a combined damage to two independent genes. To analyze the phenotypic effect of the mutations affecting the Rpt1/p48B gene alone, imprecise P-element excision mutants were selected. One of them, the pupal lethal P1 mutation, is a hypomorphic allele of the Rpt1/p48B gene, in which the displacement of two essential regulatory sequences of the gene occurred due to the insertion of a 32 bp residual P-element sequence. This mutation caused a 30-fold drop in the cellular concentration of the Rpt1/p48B mRNA. The decline in the cellular Rpt1/p48B protein concentration induced serious damage in the assembly of the 26S proteasomes, the accumulation of multiubiquitinated proteins, a change in the phosphorylation pattern of the subunit and depletion of this ATPase protein from the chromatin.

Original languageEnglish
Pages (from-to)17-29
Number of pages13
JournalMolecular Genetics and Genomics
Volume278
Issue number1
DOIs
Publication statusPublished - Jul 1 2007

Keywords

  • 26S proteasome
  • ATPase subunit
  • P-element insertion
  • Regulatory particle
  • Rpt1/p48B subunit

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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