Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath)

R. Csáki, T. Hanczár, L. Bodrossy, J. C. Murrell, K. L. Kovács

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Abstract

The first gene cluster encoding for a membrane bound [NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced. The cluster consisted of the structural genes hupS and hupL and accessory genes hupE, hupC and hupD. A ΔhupSL deletion mutant of Mc. capsulatus was constructed by marker exchange mutagenesis. Membrane associated hydrogenase activity disappeared. The membrane associated hydrogenase appeared to have a hydrogen uptake function in vivo.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalFEMS microbiology letters
Volume205
Issue number2
DOIs
Publication statusPublished - Dec 18 2001

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Keywords

  • Hup gene cluster
  • Membrane bound hydrogenase
  • Methanotrophic bacterium
  • Methylococcus capsulatus (Bath)
  • [NiFe] hydrogenase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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