Molecular characterization of rotaviruses in mid-western Turkey, 2006-2007

Mustafa Altindis, K. Bányai, Raike Kalayci, Cihangir Gulamber, Resit Koken, Teoman Apan, Esra Koçoǧlu, Aysegul Bukulmez, Yasin Yoldas, Pinar Aykurt

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Vaccines against rotaviruses are now available in numerous countries, including Turkey. As the vaccines may show various efficiencies against different type specificities and routine vaccination in infants might result in selection and immune escape of wild-type rotavirus strains, strain surveillance has been initiated before and during the vaccine introduction. We aimed to provide corresponding information on local strain prevalence in Anatolia, mid-western Turkey during the introduction of rotavirus vaccines. Stool samples positive for group A rotavirus by commercial enzyme immunoassay were subjected to reverse transcription-polymerase chain reaction based genotyping of the outer capsid antigens, VP7 and VP4, determining G and P type specificities respectively. Among 36 fully and 5 partially typeable strains we detected genotype G1, G2, and G9 VP7 specificities and genotype P[4], P[6] and P[8] VP4 specificities in 5 individual and 4 mixed combinations. The most common strain was G2P[4] (n=17), followed by G9P[8] (n=9). Other strains were G1P[8] (n=2), G2P[8] (n=2), G1+2P[8] (n=2), G9P[4] (n=1), G2+9P[8] (n=1), G4+9P[6] (n=1), and G2P[4+8] (n=1). Partially typed strains included 2 G1P[NT] and 3 G2P[NT] strains. Our data may help determine a baseline of the rotavirus genotype prevalence in Turkey and see if changes in the incidence of individual strains will be observed after routine use of vaccine.

Original languageEnglish
Pages (from-to)640-645
Number of pages6
JournalCentral European Journal of Medicine
Volume5
Issue number5
DOIs
Publication statusPublished - Oct 2010

Fingerprint

Rotavirus
Turkey
Rotavirus Vaccines
Vaccines
Genotype
Capsid
Immunoenzyme Techniques
Reverse Transcription
Vaccination
Antigens
Polymerase Chain Reaction
Incidence

Keywords

  • Genotyping
  • Reassortment
  • Rotavirus
  • Vaccine
  • VP4
  • VP7

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Molecular characterization of rotaviruses in mid-western Turkey, 2006-2007. / Altindis, Mustafa; Bányai, K.; Kalayci, Raike; Gulamber, Cihangir; Koken, Resit; Apan, Teoman; Koçoǧlu, Esra; Bukulmez, Aysegul; Yoldas, Yasin; Aykurt, Pinar.

In: Central European Journal of Medicine, Vol. 5, No. 5, 10.2010, p. 640-645.

Research output: Contribution to journalArticle

Altindis, M, Bányai, K, Kalayci, R, Gulamber, C, Koken, R, Apan, T, Koçoǧlu, E, Bukulmez, A, Yoldas, Y & Aykurt, P 2010, 'Molecular characterization of rotaviruses in mid-western Turkey, 2006-2007', Central European Journal of Medicine, vol. 5, no. 5, pp. 640-645. https://doi.org/10.2478/s11536-009-0130-6
Altindis, Mustafa ; Bányai, K. ; Kalayci, Raike ; Gulamber, Cihangir ; Koken, Resit ; Apan, Teoman ; Koçoǧlu, Esra ; Bukulmez, Aysegul ; Yoldas, Yasin ; Aykurt, Pinar. / Molecular characterization of rotaviruses in mid-western Turkey, 2006-2007. In: Central European Journal of Medicine. 2010 ; Vol. 5, No. 5. pp. 640-645.
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