Molecular characterization of Fusarium poae

L. Hornok, C. Fekete, G. Giczey

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Fusarium poae is a wide-spread cereal pathogen with the ability to produce trichothecenes. Molecular characterization of 15 geographically different strains of this fungus was performed. Electrophoretic karyotype analysis revealed the presence of five co-migrating chromosome-sized DNA molecules between 4.6 and 7.5 Mb in size; strains were uniform in this respect. On the other hand, almost all isolates contained one or two mini-chromosomes, smaller than 2.0 Mb, and marked individual differences were observed in the mini-chromosome patterns. A linear mitochondrial plasmid, 1.9 kb in size, was found in one of the strains and this plasmid showed no homology with other plasmids known in the genus Fusarium. The majority of strains harbored double-stranded RNA (dsRNA) elements with great individual differences in numbers and sizes. F. poae has a trichodiene-synthase gene with high sequence similarity (> 88 %) to the Tox5 gene of Fusarium sporotrichioides.

Original languageEnglish
Pages (from-to)23-31
Number of pages9
JournalSydowia
Volume48
Issue number1
Publication statusPublished - 1996

Fingerprint

Fusarium poae
Fusarium
plasmid
chromosome
Plasmids
Chromosomes
Individuality
plasmids
chromosomes
Trichothecenes
Aptitude
Double-Stranded RNA
gene
karyotype
Fusarium sporotrichioides
Karyotype
homology
cereal
Genes
trichothecenes

Keywords

  • Double-stranded RNA
  • Electrophoretic karyotype
  • Fusarium poae

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Molecular characterization of Fusarium poae. / Hornok, L.; Fekete, C.; Giczey, G.

In: Sydowia, Vol. 48, No. 1, 1996, p. 23-31.

Research output: Contribution to journalArticle

Hornok, L, Fekete, C & Giczey, G 1996, 'Molecular characterization of Fusarium poae', Sydowia, vol. 48, no. 1, pp. 23-31.
Hornok, L. ; Fekete, C. ; Giczey, G. / Molecular characterization of Fusarium poae. In: Sydowia. 1996 ; Vol. 48, No. 1. pp. 23-31.
@article{0cbdd9bfd2b94d20b73483d136978e6e,
title = "Molecular characterization of Fusarium poae",
abstract = "Fusarium poae is a wide-spread cereal pathogen with the ability to produce trichothecenes. Molecular characterization of 15 geographically different strains of this fungus was performed. Electrophoretic karyotype analysis revealed the presence of five co-migrating chromosome-sized DNA molecules between 4.6 and 7.5 Mb in size; strains were uniform in this respect. On the other hand, almost all isolates contained one or two mini-chromosomes, smaller than 2.0 Mb, and marked individual differences were observed in the mini-chromosome patterns. A linear mitochondrial plasmid, 1.9 kb in size, was found in one of the strains and this plasmid showed no homology with other plasmids known in the genus Fusarium. The majority of strains harbored double-stranded RNA (dsRNA) elements with great individual differences in numbers and sizes. F. poae has a trichodiene-synthase gene with high sequence similarity (> 88 {\%}) to the Tox5 gene of Fusarium sporotrichioides.",
keywords = "Double-stranded RNA, Electrophoretic karyotype, Fusarium poae",
author = "L. Hornok and C. Fekete and G. Giczey",
year = "1996",
language = "English",
volume = "48",
pages = "23--31",
journal = "Sydowia",
issn = "0082-0598",
publisher = "Verlag Ferdinand Berger und Sohne GmbH",
number = "1",

}

TY - JOUR

T1 - Molecular characterization of Fusarium poae

AU - Hornok, L.

AU - Fekete, C.

AU - Giczey, G.

PY - 1996

Y1 - 1996

N2 - Fusarium poae is a wide-spread cereal pathogen with the ability to produce trichothecenes. Molecular characterization of 15 geographically different strains of this fungus was performed. Electrophoretic karyotype analysis revealed the presence of five co-migrating chromosome-sized DNA molecules between 4.6 and 7.5 Mb in size; strains were uniform in this respect. On the other hand, almost all isolates contained one or two mini-chromosomes, smaller than 2.0 Mb, and marked individual differences were observed in the mini-chromosome patterns. A linear mitochondrial plasmid, 1.9 kb in size, was found in one of the strains and this plasmid showed no homology with other plasmids known in the genus Fusarium. The majority of strains harbored double-stranded RNA (dsRNA) elements with great individual differences in numbers and sizes. F. poae has a trichodiene-synthase gene with high sequence similarity (> 88 %) to the Tox5 gene of Fusarium sporotrichioides.

AB - Fusarium poae is a wide-spread cereal pathogen with the ability to produce trichothecenes. Molecular characterization of 15 geographically different strains of this fungus was performed. Electrophoretic karyotype analysis revealed the presence of five co-migrating chromosome-sized DNA molecules between 4.6 and 7.5 Mb in size; strains were uniform in this respect. On the other hand, almost all isolates contained one or two mini-chromosomes, smaller than 2.0 Mb, and marked individual differences were observed in the mini-chromosome patterns. A linear mitochondrial plasmid, 1.9 kb in size, was found in one of the strains and this plasmid showed no homology with other plasmids known in the genus Fusarium. The majority of strains harbored double-stranded RNA (dsRNA) elements with great individual differences in numbers and sizes. F. poae has a trichodiene-synthase gene with high sequence similarity (> 88 %) to the Tox5 gene of Fusarium sporotrichioides.

KW - Double-stranded RNA

KW - Electrophoretic karyotype

KW - Fusarium poae

UR - http://www.scopus.com/inward/record.url?scp=0030524779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030524779&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030524779

VL - 48

SP - 23

EP - 31

JO - Sydowia

JF - Sydowia

SN - 0082-0598

IS - 1

ER -