Molecular characterization of campylobacter lanienae strains isolated from food-producing animals

Nóra Schweitzer, Ivelina Damjanova, Éva Kaszanyitzky, K. Ursu, Péterné Samu, Ádám György Tóth, J. Varga, A. Dán

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

During 2008 and 2009, within the framework of the Hungarian monitoring program of antibiotic resistance of zoonotic agents from food-producing animals, a significant number (43 strains) of Campylobacter lanienae were detected for the first time in Hungary. The isolates were genotyped using partial 16S rRNA gene sequencing and pulsed-field gel electrophoresis using three different restriction enzymes. The antimicrobial resistance of the isolates was determined by microtiter broth dilution. C. lanienae isolation was successful only from swine but not from other animal species. According to phylogenetic analysis, clustering of the isolates shows the same extensive genetic diversity as other Campylobacter species. Sequence analysis of the partial 16S rRNA gene showed that additional variations exist in variable regions Vc2 and Vc6. SmaI restriction enzyme proved to be the most efficient for pulsed-field gel electrophoresis analysis of C. lanienae. A significant tetracycline resistance (60.9%) and the presence of erythromycin-, enrofloxacin-, and multiresistant C. lanienae strains were found. Although the pathogenic potential of C. lanienae in humans is currently unknown, this study demonstrates that C. lanieanae is common in pigs in the country, provides further details on the genotypic and phenotypic properties of C. lanienae, and offers a genotyping method for use in source tracing.

Original languageEnglish
Pages (from-to)615-621
Number of pages7
JournalFoodborne Pathogens and Disease
Volume8
Issue number5
DOIs
Publication statusPublished - May 1 2011

Fingerprint

Campylobacter lanienae
Campylobacter
Pulsed Field Gel Electrophoresis
rRNA Genes
Swine
Tetracycline Resistance
Food
Hungary
Zoonoses
Erythromycin
Enzymes
Microbial Drug Resistance
Sequence Analysis
Cluster Analysis
animals
pulsed-field gel electrophoresis
antibiotic resistance
ribosomal RNA
swine
enrofloxacin

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Food Science
  • Animal Science and Zoology

Cite this

Molecular characterization of campylobacter lanienae strains isolated from food-producing animals. / Schweitzer, Nóra; Damjanova, Ivelina; Kaszanyitzky, Éva; Ursu, K.; Samu, Péterné; Tóth, Ádám György; Varga, J.; Dán, A.

In: Foodborne Pathogens and Disease, Vol. 8, No. 5, 01.05.2011, p. 615-621.

Research output: Contribution to journalArticle

Schweitzer, Nóra ; Damjanova, Ivelina ; Kaszanyitzky, Éva ; Ursu, K. ; Samu, Péterné ; Tóth, Ádám György ; Varga, J. ; Dán, A. / Molecular characterization of campylobacter lanienae strains isolated from food-producing animals. In: Foodborne Pathogens and Disease. 2011 ; Vol. 8, No. 5. pp. 615-621.
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