Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes and the first adenovirus with either one short or three long fibers per penton

Judit J. Pe´nzes, Rosa Mene´ndez-Conejero, Gabriela N. Condezo, Inna Ball, Tibor Papp, Andor Doszpoly, Alberto Paradela, Ana J. Pe´rez-Berna´, Mari´a Lo´pez-Sanz, Thanh H. Nguyen, Mark J. van Raaij, Rachel E. Marschang, B. Harrach, M. Benkó, Carmen San Marti´n

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Although adenoviruses (AdVs) have been found in a wide variety of reptiles, including numerous squamate species, turtles, and crocodiles, the number of reptilian adenovirus isolates is still scarce. The only fully sequenced reptilian adenovirus, snake adenovirus 1 (SnAdV-1), belongs to the Atadenovirus genus. Recently, two new atadenoviruses were isolated from a captive Gila monster (Heloderma suspectum) and Mexican beaded lizards (Heloderma horridum). Here we report the full genomic and proteomic characterization of the latter, designated lizard adenovirus 2 (LAdV-2). The double-stranded DNA (dsDNA) genome of LAdV-2 is 32,965 bp long, with an average G+C content of 44.16%. The overall arrangement and gene content of the LAdV-2 genome were largely concordant with those in other atadenoviruses, except for four novel open reading frames (ORFs) at the right end of the genome. Phylogeny reconstructions and plesiomorphic traits shared with SnAdV-1 further supported the assignment of LAdV-2 to the Atadenovirus genus. Surprisingly, two fiber genes were found for the first time in an atadenovirus. After optimizing the production of LAdV-2 in cell culture, we determined the protein compositions of the virions. The two fiber genes produce two fiber proteins of different sizes that are incorporated into the viral particles. Interestingly, the two different fiber proteins assemble as either one short or three long fiber projections per vertex. Stoichiometry estimations indicate that the long fiber triplet is present at only one or two vertices per virion. Neither triple fibers nor a mixed number of fibers per vertex had previously been reported for adenoviruses or any other virus.

Original languageEnglish
Pages (from-to)11304-11314
Number of pages11
JournalJournal of Virology
Volume88
Issue number19
DOIs
Publication statusPublished - 2014

Fingerprint

Atadenovirus
Lizards
Adenoviridae
lizards
Genes
Snake adenovirus A
genes
virion
Virion
Snakes
genome
Genome
crocodiles
Alligators and Crocodiles
protein composition
Squamata
stoichiometry
Proteins
Turtles
Reptiles

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes and the first adenovirus with either one short or three long fibers per penton. / Pe´nzes, Judit J.; Mene´ndez-Conejero, Rosa; Condezo, Gabriela N.; Ball, Inna; Papp, Tibor; Doszpoly, Andor; Paradela, Alberto; Pe´rez-Berna´, Ana J.; Lo´pez-Sanz, Mari´a; Nguyen, Thanh H.; van Raaij, Mark J.; Marschang, Rachel E.; Harrach, B.; Benkó, M.; Marti´n, Carmen San.

In: Journal of Virology, Vol. 88, No. 19, 2014, p. 11304-11314.

Research output: Contribution to journalArticle

Pe´nzes, JJ, Mene´ndez-Conejero, R, Condezo, GN, Ball, I, Papp, T, Doszpoly, A, Paradela, A, Pe´rez-Berna´, AJ, Lo´pez-Sanz, M, Nguyen, TH, van Raaij, MJ, Marschang, RE, Harrach, B, Benkó, M & Marti´n, CS 2014, 'Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes and the first adenovirus with either one short or three long fibers per penton', Journal of Virology, vol. 88, no. 19, pp. 11304-11314. https://doi.org/10.1128/JVI.00306-14
Pe´nzes, Judit J. ; Mene´ndez-Conejero, Rosa ; Condezo, Gabriela N. ; Ball, Inna ; Papp, Tibor ; Doszpoly, Andor ; Paradela, Alberto ; Pe´rez-Berna´, Ana J. ; Lo´pez-Sanz, Mari´a ; Nguyen, Thanh H. ; van Raaij, Mark J. ; Marschang, Rachel E. ; Harrach, B. ; Benkó, M. ; Marti´n, Carmen San. / Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes and the first adenovirus with either one short or three long fibers per penton. In: Journal of Virology. 2014 ; Vol. 88, No. 19. pp. 11304-11314.
@article{a9ed3aa9ce2448c5aaaa12dfd14acdaa,
title = "Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes and the first adenovirus with either one short or three long fibers per penton",
abstract = "Although adenoviruses (AdVs) have been found in a wide variety of reptiles, including numerous squamate species, turtles, and crocodiles, the number of reptilian adenovirus isolates is still scarce. The only fully sequenced reptilian adenovirus, snake adenovirus 1 (SnAdV-1), belongs to the Atadenovirus genus. Recently, two new atadenoviruses were isolated from a captive Gila monster (Heloderma suspectum) and Mexican beaded lizards (Heloderma horridum). Here we report the full genomic and proteomic characterization of the latter, designated lizard adenovirus 2 (LAdV-2). The double-stranded DNA (dsDNA) genome of LAdV-2 is 32,965 bp long, with an average G+C content of 44.16{\%}. The overall arrangement and gene content of the LAdV-2 genome were largely concordant with those in other atadenoviruses, except for four novel open reading frames (ORFs) at the right end of the genome. Phylogeny reconstructions and plesiomorphic traits shared with SnAdV-1 further supported the assignment of LAdV-2 to the Atadenovirus genus. Surprisingly, two fiber genes were found for the first time in an atadenovirus. After optimizing the production of LAdV-2 in cell culture, we determined the protein compositions of the virions. The two fiber genes produce two fiber proteins of different sizes that are incorporated into the viral particles. Interestingly, the two different fiber proteins assemble as either one short or three long fiber projections per vertex. Stoichiometry estimations indicate that the long fiber triplet is present at only one or two vertices per virion. Neither triple fibers nor a mixed number of fibers per vertex had previously been reported for adenoviruses or any other virus.",
author = "Pe´nzes, {Judit J.} and Rosa Mene´ndez-Conejero and Condezo, {Gabriela N.} and Inna Ball and Tibor Papp and Andor Doszpoly and Alberto Paradela and Pe´rez-Berna´, {Ana J.} and Mari´a Lo´pez-Sanz and Nguyen, {Thanh H.} and {van Raaij}, {Mark J.} and Marschang, {Rachel E.} and B. Harrach and M. Benk{\'o} and Marti´n, {Carmen San}",
year = "2014",
doi = "10.1128/JVI.00306-14",
language = "English",
volume = "88",
pages = "11304--11314",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "19",

}

TY - JOUR

T1 - Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes and the first adenovirus with either one short or three long fibers per penton

AU - Pe´nzes, Judit J.

AU - Mene´ndez-Conejero, Rosa

AU - Condezo, Gabriela N.

AU - Ball, Inna

AU - Papp, Tibor

AU - Doszpoly, Andor

AU - Paradela, Alberto

AU - Pe´rez-Berna´, Ana J.

AU - Lo´pez-Sanz, Mari´a

AU - Nguyen, Thanh H.

AU - van Raaij, Mark J.

AU - Marschang, Rachel E.

AU - Harrach, B.

AU - Benkó, M.

AU - Marti´n, Carmen San

PY - 2014

Y1 - 2014

N2 - Although adenoviruses (AdVs) have been found in a wide variety of reptiles, including numerous squamate species, turtles, and crocodiles, the number of reptilian adenovirus isolates is still scarce. The only fully sequenced reptilian adenovirus, snake adenovirus 1 (SnAdV-1), belongs to the Atadenovirus genus. Recently, two new atadenoviruses were isolated from a captive Gila monster (Heloderma suspectum) and Mexican beaded lizards (Heloderma horridum). Here we report the full genomic and proteomic characterization of the latter, designated lizard adenovirus 2 (LAdV-2). The double-stranded DNA (dsDNA) genome of LAdV-2 is 32,965 bp long, with an average G+C content of 44.16%. The overall arrangement and gene content of the LAdV-2 genome were largely concordant with those in other atadenoviruses, except for four novel open reading frames (ORFs) at the right end of the genome. Phylogeny reconstructions and plesiomorphic traits shared with SnAdV-1 further supported the assignment of LAdV-2 to the Atadenovirus genus. Surprisingly, two fiber genes were found for the first time in an atadenovirus. After optimizing the production of LAdV-2 in cell culture, we determined the protein compositions of the virions. The two fiber genes produce two fiber proteins of different sizes that are incorporated into the viral particles. Interestingly, the two different fiber proteins assemble as either one short or three long fiber projections per vertex. Stoichiometry estimations indicate that the long fiber triplet is present at only one or two vertices per virion. Neither triple fibers nor a mixed number of fibers per vertex had previously been reported for adenoviruses or any other virus.

AB - Although adenoviruses (AdVs) have been found in a wide variety of reptiles, including numerous squamate species, turtles, and crocodiles, the number of reptilian adenovirus isolates is still scarce. The only fully sequenced reptilian adenovirus, snake adenovirus 1 (SnAdV-1), belongs to the Atadenovirus genus. Recently, two new atadenoviruses were isolated from a captive Gila monster (Heloderma suspectum) and Mexican beaded lizards (Heloderma horridum). Here we report the full genomic and proteomic characterization of the latter, designated lizard adenovirus 2 (LAdV-2). The double-stranded DNA (dsDNA) genome of LAdV-2 is 32,965 bp long, with an average G+C content of 44.16%. The overall arrangement and gene content of the LAdV-2 genome were largely concordant with those in other atadenoviruses, except for four novel open reading frames (ORFs) at the right end of the genome. Phylogeny reconstructions and plesiomorphic traits shared with SnAdV-1 further supported the assignment of LAdV-2 to the Atadenovirus genus. Surprisingly, two fiber genes were found for the first time in an atadenovirus. After optimizing the production of LAdV-2 in cell culture, we determined the protein compositions of the virions. The two fiber genes produce two fiber proteins of different sizes that are incorporated into the viral particles. Interestingly, the two different fiber proteins assemble as either one short or three long fiber projections per vertex. Stoichiometry estimations indicate that the long fiber triplet is present at only one or two vertices per virion. Neither triple fibers nor a mixed number of fibers per vertex had previously been reported for adenoviruses or any other virus.

UR - http://www.scopus.com/inward/record.url?scp=84906987238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906987238&partnerID=8YFLogxK

U2 - 10.1128/JVI.00306-14

DO - 10.1128/JVI.00306-14

M3 - Article

C2 - 25056898

AN - SCOPUS:84906987238

VL - 88

SP - 11304

EP - 11314

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 19

ER -