Molecular characterization and in planta detection of Sclerotinia sclerotiorum endopolygalacturonase genes

Zsolt Kasza, C. Vágvölgyi, Michel Févre, Pascale Cotton

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Sclerotinia sclerotiorum, a plant pathogenic ascomycete, secretes multiple pectinolytic enzymes that facilitate penetration, colonization, and maceration of the plant tissues. Molecular analysis has previously revealed that the pectinolytic system of the fungus is organized as a multigene family, among which a subfamily of three members encoding for neutral endopolygalacturonase (endoPG) isoforms has been characterized. Here we describe the isolation and characterization of three additional endoPG-encoding genes (pg5, pg6, and pg7) that belong to distinct phylogenetic groups. Pairwise sequence comparison between the known endoPGs from S. sclerotiorum revealed 43% to 97% identity, and the genomic organization of the pectinolytic system showed a great similarity to that of the related necrotroph Botrytis cinerea. During plant pathogenesis, a sequential expression of the endoPG-encoding genes was shown.

Original languageEnglish
Pages (from-to)208-213
Number of pages6
JournalCurrent Microbiology
Volume48
Issue number3
DOIs
Publication statusPublished - 2004

Fingerprint

Polygalacturonase
Ascomycota
Genes
Botrytis
Multigene Family
Protein Isoforms
Fungi
Enzymes

ASJC Scopus subject areas

  • Microbiology

Cite this

Molecular characterization and in planta detection of Sclerotinia sclerotiorum endopolygalacturonase genes. / Kasza, Zsolt; Vágvölgyi, C.; Févre, Michel; Cotton, Pascale.

In: Current Microbiology, Vol. 48, No. 3, 2004, p. 208-213.

Research output: Contribution to journalArticle

@article{b5c186b4457545b6829e65a5d2b65756,
title = "Molecular characterization and in planta detection of Sclerotinia sclerotiorum endopolygalacturonase genes",
abstract = "Sclerotinia sclerotiorum, a plant pathogenic ascomycete, secretes multiple pectinolytic enzymes that facilitate penetration, colonization, and maceration of the plant tissues. Molecular analysis has previously revealed that the pectinolytic system of the fungus is organized as a multigene family, among which a subfamily of three members encoding for neutral endopolygalacturonase (endoPG) isoforms has been characterized. Here we describe the isolation and characterization of three additional endoPG-encoding genes (pg5, pg6, and pg7) that belong to distinct phylogenetic groups. Pairwise sequence comparison between the known endoPGs from S. sclerotiorum revealed 43{\%} to 97{\%} identity, and the genomic organization of the pectinolytic system showed a great similarity to that of the related necrotroph Botrytis cinerea. During plant pathogenesis, a sequential expression of the endoPG-encoding genes was shown.",
author = "Zsolt Kasza and C. V{\'a}gv{\"o}lgyi and Michel F{\'e}vre and Pascale Cotton",
year = "2004",
doi = "10.1007/s00284-003-4166-6",
language = "English",
volume = "48",
pages = "208--213",
journal = "Current Microbiology",
issn = "0343-8651",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Molecular characterization and in planta detection of Sclerotinia sclerotiorum endopolygalacturonase genes

AU - Kasza, Zsolt

AU - Vágvölgyi, C.

AU - Févre, Michel

AU - Cotton, Pascale

PY - 2004

Y1 - 2004

N2 - Sclerotinia sclerotiorum, a plant pathogenic ascomycete, secretes multiple pectinolytic enzymes that facilitate penetration, colonization, and maceration of the plant tissues. Molecular analysis has previously revealed that the pectinolytic system of the fungus is organized as a multigene family, among which a subfamily of three members encoding for neutral endopolygalacturonase (endoPG) isoforms has been characterized. Here we describe the isolation and characterization of three additional endoPG-encoding genes (pg5, pg6, and pg7) that belong to distinct phylogenetic groups. Pairwise sequence comparison between the known endoPGs from S. sclerotiorum revealed 43% to 97% identity, and the genomic organization of the pectinolytic system showed a great similarity to that of the related necrotroph Botrytis cinerea. During plant pathogenesis, a sequential expression of the endoPG-encoding genes was shown.

AB - Sclerotinia sclerotiorum, a plant pathogenic ascomycete, secretes multiple pectinolytic enzymes that facilitate penetration, colonization, and maceration of the plant tissues. Molecular analysis has previously revealed that the pectinolytic system of the fungus is organized as a multigene family, among which a subfamily of three members encoding for neutral endopolygalacturonase (endoPG) isoforms has been characterized. Here we describe the isolation and characterization of three additional endoPG-encoding genes (pg5, pg6, and pg7) that belong to distinct phylogenetic groups. Pairwise sequence comparison between the known endoPGs from S. sclerotiorum revealed 43% to 97% identity, and the genomic organization of the pectinolytic system showed a great similarity to that of the related necrotroph Botrytis cinerea. During plant pathogenesis, a sequential expression of the endoPG-encoding genes was shown.

UR - http://www.scopus.com/inward/record.url?scp=2942536845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942536845&partnerID=8YFLogxK

U2 - 10.1007/s00284-003-4166-6

DO - 10.1007/s00284-003-4166-6

M3 - Article

C2 - 15057467

AN - SCOPUS:2942536845

VL - 48

SP - 208

EP - 213

JO - Current Microbiology

JF - Current Microbiology

SN - 0343-8651

IS - 3

ER -