Molecular characterization and expression of a tobacco histone H1 cDNA

Miklos Szekeres, Thomas Haizel, Eva Adam, Ferenc Nagy

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have isolated a 1104 bp tobacco cDNA clone (H1c12) which includes an 846 bp open reading frame. This encodes a polypeptide of 282 amino acid residues and represents the largest plant H1 histone identified so far. The structure of the deduced protein shows the classical tripartite organization of the H1-type linker histones. The expression of the tobacco H1 histone gene(s) corresponding to the H1c12 cDNA clone was examined during different developmental stages. We found that, at the level of steadystate mRNA, expression of gene(s) encoding this H1 histone was rapidly induced in germinating seeds. The H1 gene was expressed in all tissues examined. However, its expression was higher in tissues known to contain meristematic cells. Furthermore, in the leaves of mature plants accumulation of the H1 mRNA exhibits a very characteristic oscillation. This latter finding indicates that, at least in fully developed plants, the expression of this type of H1 histone gene(s) is modulated by a diurnal cycle.

Original languageEnglish
Pages (from-to)597-605
Number of pages9
JournalPlant molecular biology
Volume27
Issue number3
DOIs
Publication statusPublished - Feb 1 1995

Keywords

  • H1 histone genes
  • expression
  • oscillation
  • tobacco
  • transcription

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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