Molecular characterisation of multidrug-resistant Bacteroides isolates from Hungarian clinical samples

Károly Péter Sárvári, J. Sóki, Katalin Kristóf, Emese Juhász, Cecilia Miszti, Szilvia Zsóka Melegh, Krisztina Latkóczy, E. Urbán

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives: Members of the Bacteroides fragilis group are the most important components of the normal human gut microbiota, however these bacteria can also cause severe infections. Due to frequent use of antibiotics, the spread of multidrug-resistant (MDR) strains is a real threat worldwide. Methods: In a multicentre study, 400 Bacteroides isolates from five Hungarian microbiology laboratories were cultured and were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Minimum inhibitory concentrations (MICs) of ten antibiotics were determined by the agar dilution method and were evaluated according to EUCAST or CLSI breakpoints. Results: Six MDR strains were found and their antibiotic resistance genes were investigated by molecular methods The DNA amplicon of B. fragilis SZ38 was sequenced to search for a mutation in the gyrA gene. Among the six MDR isolates, one cfiA-, two cepA-, three cfxA-, two ermG-, six tetQ-, three tetX- and two bexA-positive strains were found. None of the MDR isolates harboured cepA, nim, ermB or tetX1 genes. Conclusions: In the past 12 years, only a few cases of MDR Bacteroides infections have been reported. Within a comprehensive multicentre survey, we demonstrated the relatively high prevalence of MDR strains isolated in one centre with five isolates as well as one isolate from another centre during a relatively short period of time. This study highlights the importance of antimicrobial susceptibility testing and surveillance among B. fragilis group isolates.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalJournal of Global Antimicrobial Resistance
Volume13
DOIs
Publication statusPublished - Jun 1 2018

Fingerprint

Bacteroides fragilis
Bacteroides
Bacteroides Infections
Genes
Anti-Bacterial Agents
Microbial Sensitivity Tests
Microbial Drug Resistance
Microbiology
Multicenter Studies
Agar
Mass Spectrometry
Lasers
Bacteria
Mutation
DNA
Infection

Keywords

  • Agar dilution
  • Antibiotic resistance genes
  • Bacteroides
  • Multidrug resistance
  • RT-PCR
  • Sequencing

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

Cite this

Molecular characterisation of multidrug-resistant Bacteroides isolates from Hungarian clinical samples. / Sárvári, Károly Péter; Sóki, J.; Kristóf, Katalin; Juhász, Emese; Miszti, Cecilia; Melegh, Szilvia Zsóka; Latkóczy, Krisztina; Urbán, E.

In: Journal of Global Antimicrobial Resistance, Vol. 13, 01.06.2018, p. 65-69.

Research output: Contribution to journalArticle

Sárvári, Károly Péter ; Sóki, J. ; Kristóf, Katalin ; Juhász, Emese ; Miszti, Cecilia ; Melegh, Szilvia Zsóka ; Latkóczy, Krisztina ; Urbán, E. / Molecular characterisation of multidrug-resistant Bacteroides isolates from Hungarian clinical samples. In: Journal of Global Antimicrobial Resistance. 2018 ; Vol. 13. pp. 65-69.
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AU - Sárvári, Károly Péter

AU - Sóki, J.

AU - Kristóf, Katalin

AU - Juhász, Emese

AU - Miszti, Cecilia

AU - Melegh, Szilvia Zsóka

AU - Latkóczy, Krisztina

AU - Urbán, E.

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N2 - Objectives: Members of the Bacteroides fragilis group are the most important components of the normal human gut microbiota, however these bacteria can also cause severe infections. Due to frequent use of antibiotics, the spread of multidrug-resistant (MDR) strains is a real threat worldwide. Methods: In a multicentre study, 400 Bacteroides isolates from five Hungarian microbiology laboratories were cultured and were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Minimum inhibitory concentrations (MICs) of ten antibiotics were determined by the agar dilution method and were evaluated according to EUCAST or CLSI breakpoints. Results: Six MDR strains were found and their antibiotic resistance genes were investigated by molecular methods The DNA amplicon of B. fragilis SZ38 was sequenced to search for a mutation in the gyrA gene. Among the six MDR isolates, one cfiA-, two cepA-, three cfxA-, two ermG-, six tetQ-, three tetX- and two bexA-positive strains were found. None of the MDR isolates harboured cepA, nim, ermB or tetX1 genes. Conclusions: In the past 12 years, only a few cases of MDR Bacteroides infections have been reported. Within a comprehensive multicentre survey, we demonstrated the relatively high prevalence of MDR strains isolated in one centre with five isolates as well as one isolate from another centre during a relatively short period of time. This study highlights the importance of antimicrobial susceptibility testing and surveillance among B. fragilis group isolates.

AB - Objectives: Members of the Bacteroides fragilis group are the most important components of the normal human gut microbiota, however these bacteria can also cause severe infections. Due to frequent use of antibiotics, the spread of multidrug-resistant (MDR) strains is a real threat worldwide. Methods: In a multicentre study, 400 Bacteroides isolates from five Hungarian microbiology laboratories were cultured and were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Minimum inhibitory concentrations (MICs) of ten antibiotics were determined by the agar dilution method and were evaluated according to EUCAST or CLSI breakpoints. Results: Six MDR strains were found and their antibiotic resistance genes were investigated by molecular methods The DNA amplicon of B. fragilis SZ38 was sequenced to search for a mutation in the gyrA gene. Among the six MDR isolates, one cfiA-, two cepA-, three cfxA-, two ermG-, six tetQ-, three tetX- and two bexA-positive strains were found. None of the MDR isolates harboured cepA, nim, ermB or tetX1 genes. Conclusions: In the past 12 years, only a few cases of MDR Bacteroides infections have been reported. Within a comprehensive multicentre survey, we demonstrated the relatively high prevalence of MDR strains isolated in one centre with five isolates as well as one isolate from another centre during a relatively short period of time. This study highlights the importance of antimicrobial susceptibility testing and surveillance among B. fragilis group isolates.

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