Molecular characterisation of Hungarian macrolide-resistant Streptococcus pneumoniae isolates, including three highly resistant strains with the mef gene

Orsolya Dobay, F. Rozgonyi, Sebastian G B Amyes

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The macrolide resistance of 304 Hungarian Streptococcus pneumoniae isolates was investigated. Antibiotic sensitivity testing was performed in air and in 5% CO2. More erythromycin resistance was noted when growth was in CO2. A resistance determinant was found in almost all isolates: erm(B) gene (87.4%), mef genes (9.2%) and one strain with the erm(TR) gene. This indicates that screening for carriage of resistance determinants should always be done in the presence of 5% CO2. We found three isolates with mef(E), which were highly resistant to erythromycin. These contained multiple and some novel, ribosomal mutations. The most prevalent serogroups were 6, 19 and 14. Based on the PFGE pattern, we found identity between the Hungarian isolates and two PMEN clones.

Original languageEnglish
Pages (from-to)488-495
Number of pages8
JournalInternational Journal of Antimicrobial Agents
Volume25
Issue number6
DOIs
Publication statusPublished - Jun 2005

Fingerprint

Macrolides
Streptococcus pneumoniae
Erythromycin
Genes
Clone Cells
Air
Anti-Bacterial Agents
Mutation
Growth

Keywords

  • Macrolide
  • mef
  • Mutations
  • Pneumococcus
  • Resistance determinants

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

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abstract = "The macrolide resistance of 304 Hungarian Streptococcus pneumoniae isolates was investigated. Antibiotic sensitivity testing was performed in air and in 5{\%} CO2. More erythromycin resistance was noted when growth was in CO2. A resistance determinant was found in almost all isolates: erm(B) gene (87.4{\%}), mef genes (9.2{\%}) and one strain with the erm(TR) gene. This indicates that screening for carriage of resistance determinants should always be done in the presence of 5{\%} CO2. We found three isolates with mef(E), which were highly resistant to erythromycin. These contained multiple and some novel, ribosomal mutations. The most prevalent serogroups were 6, 19 and 14. Based on the PFGE pattern, we found identity between the Hungarian isolates and two PMEN clones.",
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