Molecular biomarker candidates of acute kidney injury in zero-hour renal transplant needle biopsies

Reka Korbély, Julia Wilflingseder, Paul Perco, Alexander Kainz, R. Langer, Bernd Mayer, Rainer Oberbauer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The aim of this study was to assess gene expression levels of four biomarker candidates [lipocalin 2 (LCN2), the kidney injury molecule 1 (HAVCR1), netrin 1, and the cysteine-rich, angiogenic inducer, 61] in the tubulointerstitial and the glomerular compartment of zero-hour kidney biopsies in order to predict developing delayed graft function (DGF). Thirty-four needle kidney biopsy samples of deceased donors were manually microdissected. Relative gene expression levels were determined by real-time RT-PCR. For the validation of the biomarker candidates, we calculated a mixed model comparing kidneys with DGF, primary function and control samples from the healthy parts of tumor nephrectomies. Significant biomarker candidates were analyzed together with donor age in multivariable regression models to determine the prognostic value. Expression levels of LCN2 and HAVCR1 in the tubulointerstitium were significantly upregulated in the DGF group (LCN2: fold change = 3.78, P = 0.031 and HAVCR1: fold change = 3.44, P = 0.010). Odds ratios of both genes could not reach significance in the multivariable model together with donor age. The area under the curve of the receiver operating characteristic ranges between 0.75 and 0.83. LCN2 and HAVCR1 gene expression levels in zero-hour biopsies show potential to act as early biomarkers for DGF.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalTransplant International
Volume24
Issue number2
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Delayed Graft Function
Needle Biopsy
Acute Kidney Injury
Biomarkers
Transplants
Kidney
Gene Expression
Biopsy
Nephrectomy
ROC Curve
Area Under Curve
Cysteine
Real-Time Polymerase Chain Reaction
Odds Ratio
Lipocalin-2
Wounds and Injuries
Genes
Neoplasms

Keywords

  • acute kidney injury
  • biomarker
  • microdissection
  • qRT-PCR
  • renal transplantation

ASJC Scopus subject areas

  • Transplantation

Cite this

Molecular biomarker candidates of acute kidney injury in zero-hour renal transplant needle biopsies. / Korbély, Reka; Wilflingseder, Julia; Perco, Paul; Kainz, Alexander; Langer, R.; Mayer, Bernd; Oberbauer, Rainer.

In: Transplant International, Vol. 24, No. 2, 02.2011, p. 143-149.

Research output: Contribution to journalArticle

Korbély, Reka ; Wilflingseder, Julia ; Perco, Paul ; Kainz, Alexander ; Langer, R. ; Mayer, Bernd ; Oberbauer, Rainer. / Molecular biomarker candidates of acute kidney injury in zero-hour renal transplant needle biopsies. In: Transplant International. 2011 ; Vol. 24, No. 2. pp. 143-149.
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