Molecular biology can differentiate morphologically indistinguishable myxosporean species: Myxobolus elegans and M. hungaricus (Short communication)

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Two, morphologically indistinguishable myxosporean species, Myxobolus elegans Kashkovsky, 1966 and M. hungaricus Jaczó, 1940 were differentiated using molecular biological methods. Polymerase chain reaction (PCR) with primers specific for the family Myxobolidae was used to amplify an approximately 1600 base pairs (bp) long fragment of the 18S ribosomal RNA gene. In restriction fragment length polymorphism (RFLP) study with HinfI, MspI and TaqI enzymes, the two parasite species were easily distinguishable. The genetic distinctness was also confirmed by the DNA sequence of their PCR products. Although M. elegans and M. hungaricus are morphologically very similar, based on the results of the PCR-RFLP and the DNA sequences, we concluded that they are valid species.

Original languageEnglish
Pages (from-to)59-62
Number of pages4
JournalActa Veterinaria Hungarica
Volume50
Issue number1
Publication statusPublished - 2002

Fingerprint

Myxobolus
molecular biology
animal communication
Molecular Biology
polymerase chain reaction
Communication
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
restriction fragment length polymorphism
18S Ribosomal RNA
nucleotide sequences
rRNA Genes
Base Pairing
Parasites
ribosomal RNA
parasites
Enzymes
enzymes
genes
methodology

Keywords

  • 18S rRNA gene
  • Abramis brama
  • Leuciscus idus
  • M. hungaricus
  • Myxobolus elegans
  • Myxosporeans
  • PCR
  • RFLP

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{00d4fe237581420980523704a867f916,
title = "Molecular biology can differentiate morphologically indistinguishable myxosporean species: Myxobolus elegans and M. hungaricus (Short communication)",
abstract = "Two, morphologically indistinguishable myxosporean species, Myxobolus elegans Kashkovsky, 1966 and M. hungaricus Jacz{\'o}, 1940 were differentiated using molecular biological methods. Polymerase chain reaction (PCR) with primers specific for the family Myxobolidae was used to amplify an approximately 1600 base pairs (bp) long fragment of the 18S ribosomal RNA gene. In restriction fragment length polymorphism (RFLP) study with HinfI, MspI and TaqI enzymes, the two parasite species were easily distinguishable. The genetic distinctness was also confirmed by the DNA sequence of their PCR products. Although M. elegans and M. hungaricus are morphologically very similar, based on the results of the PCR-RFLP and the DNA sequences, we concluded that they are valid species.",
keywords = "18S rRNA gene, Abramis brama, Leuciscus idus, M. hungaricus, Myxobolus elegans, Myxosporeans, PCR, RFLP",
author = "E. Eszterbauer",
year = "2002",
language = "English",
volume = "50",
pages = "59--62",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - Molecular biology can differentiate morphologically indistinguishable myxosporean species

T2 - Myxobolus elegans and M. hungaricus (Short communication)

AU - Eszterbauer, E.

PY - 2002

Y1 - 2002

N2 - Two, morphologically indistinguishable myxosporean species, Myxobolus elegans Kashkovsky, 1966 and M. hungaricus Jaczó, 1940 were differentiated using molecular biological methods. Polymerase chain reaction (PCR) with primers specific for the family Myxobolidae was used to amplify an approximately 1600 base pairs (bp) long fragment of the 18S ribosomal RNA gene. In restriction fragment length polymorphism (RFLP) study with HinfI, MspI and TaqI enzymes, the two parasite species were easily distinguishable. The genetic distinctness was also confirmed by the DNA sequence of their PCR products. Although M. elegans and M. hungaricus are morphologically very similar, based on the results of the PCR-RFLP and the DNA sequences, we concluded that they are valid species.

AB - Two, morphologically indistinguishable myxosporean species, Myxobolus elegans Kashkovsky, 1966 and M. hungaricus Jaczó, 1940 were differentiated using molecular biological methods. Polymerase chain reaction (PCR) with primers specific for the family Myxobolidae was used to amplify an approximately 1600 base pairs (bp) long fragment of the 18S ribosomal RNA gene. In restriction fragment length polymorphism (RFLP) study with HinfI, MspI and TaqI enzymes, the two parasite species were easily distinguishable. The genetic distinctness was also confirmed by the DNA sequence of their PCR products. Although M. elegans and M. hungaricus are morphologically very similar, based on the results of the PCR-RFLP and the DNA sequences, we concluded that they are valid species.

KW - 18S rRNA gene

KW - Abramis brama

KW - Leuciscus idus

KW - M. hungaricus

KW - Myxobolus elegans

KW - Myxosporeans

KW - PCR

KW - RFLP

UR - http://www.scopus.com/inward/record.url?scp=0036361191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036361191&partnerID=8YFLogxK

M3 - Article

C2 - 12061237

AN - SCOPUS:0036361191

VL - 50

SP - 59

EP - 62

JO - Acta Veterinaria Hungarica

JF - Acta Veterinaria Hungarica

SN - 0236-6290

IS - 1

ER -