Molekuláris biológiai tesztmódszer a búza- És árpafajták fagyállóságának meghatározására

Translated title of the contribution: Molecular biological test for determining the frost tolerance of wheat and barley varieties

Research output: Contribution to journalArticle

Abstract

A molecular biological test was elaborated to determine the frost resistance of wheat and barley varieties. The method was based on detailed examinations on the effect of low temperature on the maturing process of cytoplasmic ribosomal ribonucleic acids (crRNA) in wheat and barley genotypes with different degrees of frost resistance. Preliminary results indicate that, of the precursors (pre-crRNAs) involved in the crRNA maturing process, the accumulation of the precursor with a molecular mass of 1.4 million daltons (MD) exhibited a negative correlation with the frost resistance of the wheat and barley genotypes examined, as characterised by the LT50 values. In elaborating the test, wheat and barley varieties, wheat lines and chromosome substitution lines with different degrees of frost tolerance were tested. The 1.4 MD crRNA precursor was demonstrated by means of H332PO4 labelling and separated using polyacrylamide gel electrophoresis, while the liquid scintillation technique was used to measure radioactivity.

Original languageHungarian
Pages (from-to)25-31
Number of pages7
JournalNovenytermeles
Volume48
Issue number1
Publication statusPublished - Feb 1999

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frost resistance
barley
wheat
RNA
testing
substitution lines
genotype
polyacrylamide gel electrophoresis
molecular weight
liquids
methodology
temperature

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Molekul{\'a}ris biol{\'o}giai tesztm{\'o}dszer a b{\'u}za- {\'E}s {\'a}rpafajt{\'a}k fagy{\'a}ll{\'o}s{\'a}g{\'a}nak meghat{\'a}roz{\'a}s{\'a}ra",
abstract = "A molecular biological test was elaborated to determine the frost resistance of wheat and barley varieties. The method was based on detailed examinations on the effect of low temperature on the maturing process of cytoplasmic ribosomal ribonucleic acids (crRNA) in wheat and barley genotypes with different degrees of frost resistance. Preliminary results indicate that, of the precursors (pre-crRNAs) involved in the crRNA maturing process, the accumulation of the precursor with a molecular mass of 1.4 million daltons (MD) exhibited a negative correlation with the frost resistance of the wheat and barley genotypes examined, as characterised by the LT50 values. In elaborating the test, wheat and barley varieties, wheat lines and chromosome substitution lines with different degrees of frost tolerance were tested. The 1.4 MD crRNA precursor was demonstrated by means of H332PO4 labelling and separated using polyacrylamide gel electrophoresis, while the liquid scintillation technique was used to measure radioactivity.",
author = "E. P{\'a}ldi and G. Szalai and T. Janda",
year = "1999",
month = "2",
language = "Hungarian",
volume = "48",
pages = "25--31",
journal = "Novenytermeles",
issn = "0546-8191",
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T1 - Molekuláris biológiai tesztmódszer a búza- És árpafajták fagyállóságának meghatározására

AU - Páldi, E.

AU - Szalai, G.

AU - Janda, T.

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AB - A molecular biological test was elaborated to determine the frost resistance of wheat and barley varieties. The method was based on detailed examinations on the effect of low temperature on the maturing process of cytoplasmic ribosomal ribonucleic acids (crRNA) in wheat and barley genotypes with different degrees of frost resistance. Preliminary results indicate that, of the precursors (pre-crRNAs) involved in the crRNA maturing process, the accumulation of the precursor with a molecular mass of 1.4 million daltons (MD) exhibited a negative correlation with the frost resistance of the wheat and barley genotypes examined, as characterised by the LT50 values. In elaborating the test, wheat and barley varieties, wheat lines and chromosome substitution lines with different degrees of frost tolerance were tested. The 1.4 MD crRNA precursor was demonstrated by means of H332PO4 labelling and separated using polyacrylamide gel electrophoresis, while the liquid scintillation technique was used to measure radioactivity.

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