Molekuláris biológiai tesztmódszer a búza- És árpafajták fagyállóságának meghatározására

Translated title of the contribution: Molecular biological test for determining the frost tolerance of wheat and barley varieties

Research output: Contribution to journalArticle

Abstract

A molecular biological test was elaborated to determine the frost resistance of wheat and barley varieties. The method was based on detailed examinations on the effect of low temperature on the maturing process of cytoplasmic ribosomal ribonucleic acids (crRNA) in wheat and barley genotypes with different degrees of frost resistance. Preliminary results indicate that, of the precursors (pre-crRNAs) involved in the crRNA maturing process, the accumulation of the precursor with a molecular mass of 1.4 million daltons (MD) exhibited a negative correlation with the frost resistance of the wheat and barley genotypes examined, as characterised by the LT50 values. In elaborating the test, wheat and barley varieties, wheat lines and chromosome substitution lines with different degrees of frost tolerance were tested. The 1.4 MD crRNA precursor was demonstrated by means of H332PO4 labelling and separated using polyacrylamide gel electrophoresis, while the liquid scintillation technique was used to measure radioactivity.

Translated title of the contributionMolecular biological test for determining the frost tolerance of wheat and barley varieties
Original languageHungarian
Pages (from-to)25-31
Number of pages7
JournalNovenytermeles
Volume48
Issue number1
Publication statusPublished - Feb 1 1999

ASJC Scopus subject areas

  • Agronomy and Crop Science

Fingerprint Dive into the research topics of 'Molecular biological test for determining the frost tolerance of wheat and barley varieties'. Together they form a unique fingerprint.

  • Cite this