Molecular basis of bilirubin UDP-glucuronosyltransferase induction in spontaneously diabetic rats, acetone-treated rats and starved rats

L. Braun, Marcus J. Coffey, Ferenc Puskás, T. Kardon, Gábor Nagy, Abigail A. Conley, Brian Burchell, J. Mandl

Research output: Contribution to journalArticle

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Abstract

The co-ordinated induction of several hepatic drug-metabolizing enzymes is a common feature in the regulation of drug biotransformation under normal and pathological conditions. In the present study the activity and expression of bilirubin UDP-glucuronosyltransferase (UGT1A1) were investigated in livers of BioBreeding/Worcester diabetic, fasted and acetone-treated rats. Bilirubin glucuronidation was stimulated by all three treatments; this was correlated with an increase in the UGT1A1 protein concentration in hepatic microsomes. Transcriptional induction of UGT1A1 was also observed in diabetes and starvation but not with acetone treatment, which apparently caused translational stabilization of the enzyme protein. The hormonal/metabolic alterations in diabetes and starvation might be a model for postnatal development. The sudden interruption of maternal glucose supply signals the enhanced expression of UGT1A1, giving a novel explanation for the physiological induction of bilirubin glucuronidation in newborn infants.

Original languageEnglish
Pages (from-to)587-592
Number of pages6
JournalBiochemical Journal
Volume336
Issue number3
Publication statusPublished - Dec 15 1998

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bilirubin glucuronoside glucuronosyltransferase
Medical problems
Acetone
Bilirubin
Rats
Starvation
Liver
Enzymes
Pharmaceutical Preparations
Proteins
Stabilization
Drug and Narcotic Control
Biotransformation
Microsomes
Glucose
Mothers
Newborn Infant
Therapeutics

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular basis of bilirubin UDP-glucuronosyltransferase induction in spontaneously diabetic rats, acetone-treated rats and starved rats. / Braun, L.; Coffey, Marcus J.; Puskás, Ferenc; Kardon, T.; Nagy, Gábor; Conley, Abigail A.; Burchell, Brian; Mandl, J.

In: Biochemical Journal, Vol. 336, No. 3, 15.12.1998, p. 587-592.

Research output: Contribution to journalArticle

Braun, L. ; Coffey, Marcus J. ; Puskás, Ferenc ; Kardon, T. ; Nagy, Gábor ; Conley, Abigail A. ; Burchell, Brian ; Mandl, J. / Molecular basis of bilirubin UDP-glucuronosyltransferase induction in spontaneously diabetic rats, acetone-treated rats and starved rats. In: Biochemical Journal. 1998 ; Vol. 336, No. 3. pp. 587-592.
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