Molecular and phenotypic characterization of Aspergillus japonicus and Aspergillus aculeatus strains with special regard to their mitochondrial DNA polymorphisms

Zsuzsanna Hamari, Ferenc Kevei, Éva Kovács, János Varga, Zofia Kozakiewicz, James H. Croft

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Forty Aspergillus japonicus and A. aculeatus strains, most of them wild-type isolates, were examined using various molecular and phenotypic techniques. The rDNAs proved to be invariable (even strains of the species A. aculeatus exhibited the same restriction profile), while the strains could be classified into seven different mtDNA RFLP groups. Hybridisation data suggest that six of these mtDNA types have certain common restriction sites, while mtDNA type 7, which was exhibited by some A. aculeatus strains, probably has quite different mtDNA organisation and their size was smallest among the strains studied. The RAPD technique and isoenzyme analysis revealed some variabilities within these RFLP groups and strain specific features could also be recognised. Carbon source assimilation spectra were found to be very distinctive for strains of A. japonicus, A. aculeatus and A. niger, providing a useful tool for pre-characterising new wild-type isolates of black Aspergilli. Only a limited correlation was observed between the dendrograms based on genotypic and phenotypic characters.

Original languageEnglish
Pages (from-to)337-347
Number of pages11
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume72
Issue number4
DOIs
Publication statusPublished - Dec 17 1997

Keywords

  • A. aculeatus
  • Aspergillus japonicus
  • Assimilation spectra
  • Isoenzyme
  • RAPD
  • RFLPs
  • mtDNA
  • rDNA

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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