Molecular and microscopic diversity of planktonic eukaryotes in the oligotrophic Lake Stechlin (Germany)

W. Luo, C. Bock, H. R. Li, J. Padisák, L. Krienitz

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The aim of this study was to compare a molecular and a microscopic approach to study the planktonic eukaryotic diversity of an oligotrophic lake. Plankton samples from the temperate Lake Stechlin were assessed in winter and summer 2008 by comparison of 18S rRNA gene clone libraries to light microscopic evaluations. For both approaches identical samples were used. There were remarkable differences between the main groups recovered by the contrasting methods. The microscopic analyses showed predominance of autotrophic planktonic organisms, whereas most of them could not be discovered by the molecular method which resulted in a higher diversity of heterotrophic flagellates. The microscopic survey revealed high diversity of Chlorophyta and Cryptophyta as well as the Stramenopiles groups of Bacillariophyceae and Chrysophyceae. The clone libraries, based on full-length 18S rRNA gene sequences, displayed highest diversity of Alveolata belonging to seven different subclades. Notably, Antarctic Dinophyta-related clones were detected. The occurrence of the marine phagotrophic flagellate Telonema was also documented. Comparing the two sampling seasons, rich diversity suggests that flagellates played an important role in late winter (February), however, there is relatively low diversity in summer (August). The newly discovered molecular diversity of planktonic eukaryotes in Stechlin will help to understand the biodiversity patterns in freshwater lakes.

Original languageEnglish
Pages (from-to)133-143
Number of pages11
JournalHydrobiologia
Volume661
Issue number1
DOIs
Publication statusPublished - 2011

Fingerprint

flagellate
eukaryote
eukaryotic cells
clone
Germany
clones
lakes
lake
ribosomal RNA
Chrysophyceae
winter
Bacillariophyceae
gene
summer
autotrophs
sampling
Chlorophyta
plankton
biodiversity
nucleotide sequences

Keywords

  • Lake Stechlin
  • Microbial eukaryotes
  • Microscopy
  • Molecular biodiversity

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Molecular and microscopic diversity of planktonic eukaryotes in the oligotrophic Lake Stechlin (Germany). / Luo, W.; Bock, C.; Li, H. R.; Padisák, J.; Krienitz, L.

In: Hydrobiologia, Vol. 661, No. 1, 2011, p. 133-143.

Research output: Contribution to journalArticle

Luo, W. ; Bock, C. ; Li, H. R. ; Padisák, J. ; Krienitz, L. / Molecular and microscopic diversity of planktonic eukaryotes in the oligotrophic Lake Stechlin (Germany). In: Hydrobiologia. 2011 ; Vol. 661, No. 1. pp. 133-143.
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