Molecular analysis of the sodium/ iodide symporter

Impact on thyroid and extrathyroid pathophysiology

Antonio De La Vieja, Orsolya Dohan, Orlie Levy, Nancy Carrasco

Research output: Contribution to journalArticle

258 Citations (Scopus)

Abstract

The Na+I- symporter (NIS) is an intrinsic membrane protein that mediates the active transport of iodide into the thyroid and other tissues, such as salivary glands, gastric mucosa, and lactating mammary gland. NIS plays key roles in thyroid pathophysiology as the route by which iodide reaches the gland for thyroid hormone biosynthesis and as a means for diagnostic scintigraphic imaging and for radioiodide therapy in hyperthyroidism and thyroid cancer. The molecular characterization of NIS started with the 1996 isolation of a cDNA encoding rat NIS and has since continued at a rapid pace. Anti-NIS antibodies have been prepared and used to study NIS topology and its secondary structure. The biogenesis and posttranslational modifications of NIS have been examined, a thorough electrophysiological analysis of NIS has been conducted, the cDNA encoding human NIS (hNIS) has been isolated, the genomic organization of hNIS has been elucidated, the regulation of NIS by thyrotropin and I- has been analyzed, the regulation of NIS transcription has been studied, spontaneous NIS mutations have been identified as causes of congenital iodide transport defect resulting in hypothyroidism, the roles of NIS in thyroid cancer and thyroid autoimmune disease have been examined, and the expression and regulation of NIS in extrathyroidal tissues have been investigated. In gene therapy experiments, the rat NIS gene has been transduced into various types of human cells, which then exhibited active iodide transport and became susceptible to destruction with radioiodide. The continued molecular analysis of NIS clearly holds the potential of an even greater impact on a wide spectrum of fields, ranging from structure/function of transport proteins to the diagnosis and treatment of cancer, both in the thyroid and beyond.

Original languageEnglish
Pages (from-to)1083-1105
Number of pages23
JournalPhysiological Reviews
Volume80
Issue number3
Publication statusPublished - Jul 2000

Fingerprint

Symporters
Thyroid Gland
Iodides
Thyroid Neoplasms
sodium-iodide symporter
Active Biological Transport
Complementary DNA
Thyroid Diseases

ASJC Scopus subject areas

  • Physiology

Cite this

Molecular analysis of the sodium/ iodide symporter : Impact on thyroid and extrathyroid pathophysiology. / De La Vieja, Antonio; Dohan, Orsolya; Levy, Orlie; Carrasco, Nancy.

In: Physiological Reviews, Vol. 80, No. 3, 07.2000, p. 1083-1105.

Research output: Contribution to journalArticle

De La Vieja, Antonio ; Dohan, Orsolya ; Levy, Orlie ; Carrasco, Nancy. / Molecular analysis of the sodium/ iodide symporter : Impact on thyroid and extrathyroid pathophysiology. In: Physiological Reviews. 2000 ; Vol. 80, No. 3. pp. 1083-1105.
@article{cfe3ec9353004494ad5d0ab37f4be2fe,
title = "Molecular analysis of the sodium/ iodide symporter: Impact on thyroid and extrathyroid pathophysiology",
abstract = "The Na+I- symporter (NIS) is an intrinsic membrane protein that mediates the active transport of iodide into the thyroid and other tissues, such as salivary glands, gastric mucosa, and lactating mammary gland. NIS plays key roles in thyroid pathophysiology as the route by which iodide reaches the gland for thyroid hormone biosynthesis and as a means for diagnostic scintigraphic imaging and for radioiodide therapy in hyperthyroidism and thyroid cancer. The molecular characterization of NIS started with the 1996 isolation of a cDNA encoding rat NIS and has since continued at a rapid pace. Anti-NIS antibodies have been prepared and used to study NIS topology and its secondary structure. The biogenesis and posttranslational modifications of NIS have been examined, a thorough electrophysiological analysis of NIS has been conducted, the cDNA encoding human NIS (hNIS) has been isolated, the genomic organization of hNIS has been elucidated, the regulation of NIS by thyrotropin and I- has been analyzed, the regulation of NIS transcription has been studied, spontaneous NIS mutations have been identified as causes of congenital iodide transport defect resulting in hypothyroidism, the roles of NIS in thyroid cancer and thyroid autoimmune disease have been examined, and the expression and regulation of NIS in extrathyroidal tissues have been investigated. In gene therapy experiments, the rat NIS gene has been transduced into various types of human cells, which then exhibited active iodide transport and became susceptible to destruction with radioiodide. The continued molecular analysis of NIS clearly holds the potential of an even greater impact on a wide spectrum of fields, ranging from structure/function of transport proteins to the diagnosis and treatment of cancer, both in the thyroid and beyond.",
author = "{De La Vieja}, Antonio and Orsolya Dohan and Orlie Levy and Nancy Carrasco",
year = "2000",
month = "7",
language = "English",
volume = "80",
pages = "1083--1105",
journal = "Physiological Reviews",
issn = "0031-9333",
publisher = "American Physiological Society",
number = "3",

}

TY - JOUR

T1 - Molecular analysis of the sodium/ iodide symporter

T2 - Impact on thyroid and extrathyroid pathophysiology

AU - De La Vieja, Antonio

AU - Dohan, Orsolya

AU - Levy, Orlie

AU - Carrasco, Nancy

PY - 2000/7

Y1 - 2000/7

N2 - The Na+I- symporter (NIS) is an intrinsic membrane protein that mediates the active transport of iodide into the thyroid and other tissues, such as salivary glands, gastric mucosa, and lactating mammary gland. NIS plays key roles in thyroid pathophysiology as the route by which iodide reaches the gland for thyroid hormone biosynthesis and as a means for diagnostic scintigraphic imaging and for radioiodide therapy in hyperthyroidism and thyroid cancer. The molecular characterization of NIS started with the 1996 isolation of a cDNA encoding rat NIS and has since continued at a rapid pace. Anti-NIS antibodies have been prepared and used to study NIS topology and its secondary structure. The biogenesis and posttranslational modifications of NIS have been examined, a thorough electrophysiological analysis of NIS has been conducted, the cDNA encoding human NIS (hNIS) has been isolated, the genomic organization of hNIS has been elucidated, the regulation of NIS by thyrotropin and I- has been analyzed, the regulation of NIS transcription has been studied, spontaneous NIS mutations have been identified as causes of congenital iodide transport defect resulting in hypothyroidism, the roles of NIS in thyroid cancer and thyroid autoimmune disease have been examined, and the expression and regulation of NIS in extrathyroidal tissues have been investigated. In gene therapy experiments, the rat NIS gene has been transduced into various types of human cells, which then exhibited active iodide transport and became susceptible to destruction with radioiodide. The continued molecular analysis of NIS clearly holds the potential of an even greater impact on a wide spectrum of fields, ranging from structure/function of transport proteins to the diagnosis and treatment of cancer, both in the thyroid and beyond.

AB - The Na+I- symporter (NIS) is an intrinsic membrane protein that mediates the active transport of iodide into the thyroid and other tissues, such as salivary glands, gastric mucosa, and lactating mammary gland. NIS plays key roles in thyroid pathophysiology as the route by which iodide reaches the gland for thyroid hormone biosynthesis and as a means for diagnostic scintigraphic imaging and for radioiodide therapy in hyperthyroidism and thyroid cancer. The molecular characterization of NIS started with the 1996 isolation of a cDNA encoding rat NIS and has since continued at a rapid pace. Anti-NIS antibodies have been prepared and used to study NIS topology and its secondary structure. The biogenesis and posttranslational modifications of NIS have been examined, a thorough electrophysiological analysis of NIS has been conducted, the cDNA encoding human NIS (hNIS) has been isolated, the genomic organization of hNIS has been elucidated, the regulation of NIS by thyrotropin and I- has been analyzed, the regulation of NIS transcription has been studied, spontaneous NIS mutations have been identified as causes of congenital iodide transport defect resulting in hypothyroidism, the roles of NIS in thyroid cancer and thyroid autoimmune disease have been examined, and the expression and regulation of NIS in extrathyroidal tissues have been investigated. In gene therapy experiments, the rat NIS gene has been transduced into various types of human cells, which then exhibited active iodide transport and became susceptible to destruction with radioiodide. The continued molecular analysis of NIS clearly holds the potential of an even greater impact on a wide spectrum of fields, ranging from structure/function of transport proteins to the diagnosis and treatment of cancer, both in the thyroid and beyond.

UR - http://www.scopus.com/inward/record.url?scp=0033922682&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033922682&partnerID=8YFLogxK

M3 - Article

VL - 80

SP - 1083

EP - 1105

JO - Physiological Reviews

JF - Physiological Reviews

SN - 0031-9333

IS - 3

ER -