Molecular analysis of the simultaneous production of two SHV-type extended-spectrum beta-lactamases in a clinical isolate of Enterobacter cloacae by using single-nucleotide polymorphism genotyping

D. Szabó, Melissa A. Melan, Andrea M. Hujer, Robert A. Bonomo, Kristine M. Hujer, Christopher R. Bethel, Katalin Kristóf, David L. Paterson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Bacteria that simultaneously produce multiple extended-spectrum beta-lactamases are frequently isolated. We report an Enterobacter cloacae isolate, ES24, producing four different beta-lactamases (AmpC type beta-lactamase, TEM-1, SHV-7, and a novel extended-spectrum beta-lactamase, SHV-30). Direct sequencing of P blaSHV gene products gave a "double peak" at position 703, suggesting the presence of more than one allele. Using fluorescence resonance energy transfer real-time PCR to detect single-nucleotide polymorphisms, we were able to distinguish two different blaSHV genes in a single isolate. This may prove to be a useful technique in surveys of beta-lactamase production in contemporary clinical isolates.

Original languageEnglish
Pages (from-to)4716-4720
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume49
Issue number11
DOIs
Publication statusPublished - Nov 2005

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Enterobacter cloacae
beta-Lactamases
Single Nucleotide Polymorphism
Fluorescence Resonance Energy Transfer
Genes
Real-Time Polymerase Chain Reaction
Alleles
Bacteria
AmpC beta-lactamases

ASJC Scopus subject areas

  • Pharmacology (medical)

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Molecular analysis of the simultaneous production of two SHV-type extended-spectrum beta-lactamases in a clinical isolate of Enterobacter cloacae by using single-nucleotide polymorphism genotyping. / Szabó, D.; Melan, Melissa A.; Hujer, Andrea M.; Bonomo, Robert A.; Hujer, Kristine M.; Bethel, Christopher R.; Kristóf, Katalin; Paterson, David L.

In: Antimicrobial Agents and Chemotherapy, Vol. 49, No. 11, 11.2005, p. 4716-4720.

Research output: Contribution to journalArticle

Szabó, D. ; Melan, Melissa A. ; Hujer, Andrea M. ; Bonomo, Robert A. ; Hujer, Kristine M. ; Bethel, Christopher R. ; Kristóf, Katalin ; Paterson, David L. / Molecular analysis of the simultaneous production of two SHV-type extended-spectrum beta-lactamases in a clinical isolate of Enterobacter cloacae by using single-nucleotide polymorphism genotyping. In: Antimicrobial Agents and Chemotherapy. 2005 ; Vol. 49, No. 11. pp. 4716-4720.
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