Molecular analysis of the simultaneous production of two SHV-type extended-spectrum beta-lactamases in a clinical isolate of Enterobacter cloacae by using single-nucleotide polymorphism genotyping

Dóra Szabó, Melissa A. Melan, Andrea M. Hujer, Robert A. Bonomo, Kristine M. Hujer, Christopher R. Bethel, Katalin Kristóf, David L. Paterson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Bacteria that simultaneously produce multiple extended-spectrum beta-lactamases are frequently isolated. We report an Enterobacter cloacae isolate, ES24, producing four different beta-lactamases (AmpC type beta-lactamase, TEM-1, SHV-7, and a novel extended-spectrum beta-lactamase, SHV-30). Direct sequencing of P blaSHV gene products gave a "double peak" at position 703, suggesting the presence of more than one allele. Using fluorescence resonance energy transfer real-time PCR to detect single-nucleotide polymorphisms, we were able to distinguish two different blaSHV genes in a single isolate. This may prove to be a useful technique in surveys of beta-lactamase production in contemporary clinical isolates.

Original languageEnglish
Pages (from-to)4716-4720
Number of pages5
JournalAntimicrobial agents and chemotherapy
Volume49
Issue number11
DOIs
Publication statusPublished - Nov 2005

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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