Molecular analysis of S-haplotypes in peach, a self-compatible Prunus species

A. Hegedüs, Zoltán Szabó, József Nyéki, Júlia Halász, A. Pedryc

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The most commercially grown peach [Prunus persica (L.) Batsch.] cultivars do not require cross-pollination for reasonable fruit set; however, self-incompatibility is a well-known feature within the Prunoideae subfamily. Isoelectric focusing and native polyacrylamide gel electrophoresis of S-ribonucleases; PCR analyses of S-RNase and S-haplotype-specific F-box genes as well as DNA sequencing were carried out to survey the self-(in)compatibility allele pool and to uncover the nature of self-compatibility in peach. From 25 cultivars and hybrids with considerable diversity in phenotype and origin, only two S-haplotypes were detected. Allele identity could be checked by exact length determination of the PCR-amplified fragments and/or partial sequencing of the peach S1-, S2-, and Prunus davidiana (Carr.) Franch. S1-RNases. S-RNases of peach were detected to possess ribonuclease activity, and a single nucleotide polymorphism in the S1-RNase was shown, which represents a synonymous substitution and does not change the amino acid present at the position in the protein. A 700-bp fragment of the peach SFB gene was PCR-amplified, which is similar to the fragment size of functional Prunus L. SFBs. All data obtained in this study may support the contribution of genes outside the S-locus to the self-compatible phenotype of peaches.

Original languageEnglish
Pages (from-to)738-743
Number of pages6
JournalJournal of the American Society for Horticultural Science
Volume131
Issue number6
Publication statusPublished - Nov 2006

Fingerprint

Prunus
Haplotypes
peaches
haplotypes
ribonucleases
Polymerase Chain Reaction
Prunus davidiana
alleles
phenotype
Alleles
genes
Genes
Phenotype
cross pollination
Native Polyacrylamide Gel Electrophoresis
Prunus persica
isoelectric focusing
cultivars
Pollination
fruit set

Keywords

  • DNA sequencing
  • F-box
  • Modifier genes
  • Non-equilibrium pH gradient electrofocusing
  • PCR analysis
  • Prunus persica
  • Ribonuclease
  • S-allele

ASJC Scopus subject areas

  • Horticulture
  • Plant Science

Cite this

Molecular analysis of S-haplotypes in peach, a self-compatible Prunus species. / Hegedüs, A.; Szabó, Zoltán; Nyéki, József; Halász, Júlia; Pedryc, A.

In: Journal of the American Society for Horticultural Science, Vol. 131, No. 6, 11.2006, p. 738-743.

Research output: Contribution to journalArticle

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