Molecular analysis and MIRU-VNTR typing of Mycobacterium avium subsp. avium, 'hominissuis' and silvaticum strains of veterinary origin

Zsuzsanna Rónai, Ágnes Csivincsik, A. Dán, Miklós Gyuranecz

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Besides Mycobacterium avium subsp. paratuberculosis (MAP), M. avium subsp. avium (MAA), M. avium subsp. silvaticum (MAS), and 'M. avium subsp. hominissuis' (MAH) are equally important members of M. avium complex, with worldwide distribution and zoonotic potential. Genotypic discrimination is a prerequisite to epidemiological studies which can facilitate disease prevention through revealing infection sources and transmission routes. The primary aim of this study was to identify the genetic diversity within 135 MAA, 62 MAS, and 84 MAH strains isolated from wild and domestic mammals, reptiles and birds.Strains were tested for the presence of large sequence polymorphism LSPA17 and were submitted to Mycobacterial interspersed repetitive units-variable-number tandem repeat (MIRU-VNTR) analysis at 8 loci, including MIRU1, 2, 3, and 4, VNTR25, 32, and 259, and MATR9. In 12 strains hsp65 sequence code type was also determined.LSPA17 was present only in 19.9% of the strains. All LSPA17 positive strains belonged to subspecies MAH. The discriminatory power of the MIRU-VNTR loci set used reached 0.9228. Altogether 54 different genotypes were detected. Within MAH, MAA, and MAS strains 33, 16, and 5 different genotypes were observed. The described genotypes were not restricted to geographic regions or host species, but proved to be subspecies specific.Our knowledge about MAS is limited due to isolation and identification difficulties. This is the first study including a large number of MAS field strains. Our results demonstrate the high diversity of MAH and MAA strains and the relative uniformity of MAS strains.

Original languageEnglish
Pages (from-to)192-199
Number of pages8
JournalInfection, Genetics and Evolution
Volume40
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Mycobacterium avium subsp. silvaticum
Mycobacterium avium subsp. avium
Mycobacterium avium
Minisatellite Repeats
MAS
minisatellite repeats
molecular analysis
Genotype
Mycobacterium avium subsp. paratuberculosis
Mycobacterium avium Complex
genotype
Infectious Disease Transmission
Reptiles
Zoonoses
subspecies
Birds
Epidemiologic Studies
Mammals
reptile
Mycobacterium avium complex

Keywords

  • Genetic diversity
  • Hominissuis
  • LSP17
  • MIRU-VNTR
  • Mycobacterium avium subsp. avium
  • Silvaticum

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Molecular analysis and MIRU-VNTR typing of Mycobacterium avium subsp. avium, 'hominissuis' and silvaticum strains of veterinary origin. / Rónai, Zsuzsanna; Csivincsik, Ágnes; Dán, A.; Gyuranecz, Miklós.

In: Infection, Genetics and Evolution, Vol. 40, 01.06.2016, p. 192-199.

Research output: Contribution to journalArticle

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abstract = "Besides Mycobacterium avium subsp. paratuberculosis (MAP), M. avium subsp. avium (MAA), M. avium subsp. silvaticum (MAS), and 'M. avium subsp. hominissuis' (MAH) are equally important members of M. avium complex, with worldwide distribution and zoonotic potential. Genotypic discrimination is a prerequisite to epidemiological studies which can facilitate disease prevention through revealing infection sources and transmission routes. The primary aim of this study was to identify the genetic diversity within 135 MAA, 62 MAS, and 84 MAH strains isolated from wild and domestic mammals, reptiles and birds.Strains were tested for the presence of large sequence polymorphism LSPA17 and were submitted to Mycobacterial interspersed repetitive units-variable-number tandem repeat (MIRU-VNTR) analysis at 8 loci, including MIRU1, 2, 3, and 4, VNTR25, 32, and 259, and MATR9. In 12 strains hsp65 sequence code type was also determined.LSPA17 was present only in 19.9{\%} of the strains. All LSPA17 positive strains belonged to subspecies MAH. The discriminatory power of the MIRU-VNTR loci set used reached 0.9228. Altogether 54 different genotypes were detected. Within MAH, MAA, and MAS strains 33, 16, and 5 different genotypes were observed. The described genotypes were not restricted to geographic regions or host species, but proved to be subspecies specific.Our knowledge about MAS is limited due to isolation and identification difficulties. This is the first study including a large number of MAS field strains. Our results demonstrate the high diversity of MAH and MAA strains and the relative uniformity of MAS strains.",
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