MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring

Ferenc Jankovics, Rita Sinka, Tamás Lukácsovich, M. Erdélyi

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

In Drosophila, development of the embryonic germ cells depends on posterior transport and site-specific translation of oskar (osk) mRNA and on interdependent anchoring of the osk mRNA and protein within the posterior subcortical region of the oocyte [1-3]. Transport of the osk mRNA is mediated by microtubules, while anchoring of the osk gene products at the posterior pole of the oocyte is suggested to be microfilament dependent [4-7]. To date, only a single actin binding protein (Tropomyosinll) has been identified with a putative role in osk mRNA and protein anchoring [4]. This communication demonstrates that mutations in the Drosophila moesin (Dmoe) gene that encodes another actin binding protein result in delocalization of osk mRNA and protein from the posterior subcortical region and, as a consequence, in failure of embryonic germ cell development. In Dmoe mutant oocytes, the subcortical actin network is detached from the cell membrane, while the polarized microtubule cytoskeleton is unaffected. In line with the earlier observations [6, 8], colocalization of ectopic actin and OSK protein in Dmoe mutants suggests that the actin cytoskeleton anchors OSK protein to the subcortical cytoplasmic area of the Drosophila oocyte.

Original languageEnglish
Pages (from-to)2060-2065
Number of pages6
JournalCurrent Biology
Volume12
Issue number23
DOIs
Publication statusPublished - Dec 2002

Fingerprint

Cell membranes
Drosophila
Oocytes
actin
cell membranes
Actins
oocytes
microfilament proteins
Cell Membrane
Messenger RNA
Microfilament Proteins
Proteins
microfilaments
Actin Cytoskeleton
proteins
Microtubules
microtubules
germ cells
Genes
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring. / Jankovics, Ferenc; Sinka, Rita; Lukácsovich, Tamás; Erdélyi, M.

In: Current Biology, Vol. 12, No. 23, 12.2002, p. 2060-2065.

Research output: Contribution to journalArticle

Jankovics, Ferenc ; Sinka, Rita ; Lukácsovich, Tamás ; Erdélyi, M. / MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring. In: Current Biology. 2002 ; Vol. 12, No. 23. pp. 2060-2065.
@article{eefaacc362a240e994f04f19b4036ccf,
title = "MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring",
abstract = "In Drosophila, development of the embryonic germ cells depends on posterior transport and site-specific translation of oskar (osk) mRNA and on interdependent anchoring of the osk mRNA and protein within the posterior subcortical region of the oocyte [1-3]. Transport of the osk mRNA is mediated by microtubules, while anchoring of the osk gene products at the posterior pole of the oocyte is suggested to be microfilament dependent [4-7]. To date, only a single actin binding protein (Tropomyosinll) has been identified with a putative role in osk mRNA and protein anchoring [4]. This communication demonstrates that mutations in the Drosophila moesin (Dmoe) gene that encodes another actin binding protein result in delocalization of osk mRNA and protein from the posterior subcortical region and, as a consequence, in failure of embryonic germ cell development. In Dmoe mutant oocytes, the subcortical actin network is detached from the cell membrane, while the polarized microtubule cytoskeleton is unaffected. In line with the earlier observations [6, 8], colocalization of ectopic actin and OSK protein in Dmoe mutants suggests that the actin cytoskeleton anchors OSK protein to the subcortical cytoplasmic area of the Drosophila oocyte.",
author = "Ferenc Jankovics and Rita Sinka and Tam{\'a}s Luk{\'a}csovich and M. Erd{\'e}lyi",
year = "2002",
month = "12",
doi = "10.1016/S0960-9822(02)01256-3",
language = "English",
volume = "12",
pages = "2060--2065",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "23",

}

TY - JOUR

T1 - MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring

AU - Jankovics, Ferenc

AU - Sinka, Rita

AU - Lukácsovich, Tamás

AU - Erdélyi, M.

PY - 2002/12

Y1 - 2002/12

N2 - In Drosophila, development of the embryonic germ cells depends on posterior transport and site-specific translation of oskar (osk) mRNA and on interdependent anchoring of the osk mRNA and protein within the posterior subcortical region of the oocyte [1-3]. Transport of the osk mRNA is mediated by microtubules, while anchoring of the osk gene products at the posterior pole of the oocyte is suggested to be microfilament dependent [4-7]. To date, only a single actin binding protein (Tropomyosinll) has been identified with a putative role in osk mRNA and protein anchoring [4]. This communication demonstrates that mutations in the Drosophila moesin (Dmoe) gene that encodes another actin binding protein result in delocalization of osk mRNA and protein from the posterior subcortical region and, as a consequence, in failure of embryonic germ cell development. In Dmoe mutant oocytes, the subcortical actin network is detached from the cell membrane, while the polarized microtubule cytoskeleton is unaffected. In line with the earlier observations [6, 8], colocalization of ectopic actin and OSK protein in Dmoe mutants suggests that the actin cytoskeleton anchors OSK protein to the subcortical cytoplasmic area of the Drosophila oocyte.

AB - In Drosophila, development of the embryonic germ cells depends on posterior transport and site-specific translation of oskar (osk) mRNA and on interdependent anchoring of the osk mRNA and protein within the posterior subcortical region of the oocyte [1-3]. Transport of the osk mRNA is mediated by microtubules, while anchoring of the osk gene products at the posterior pole of the oocyte is suggested to be microfilament dependent [4-7]. To date, only a single actin binding protein (Tropomyosinll) has been identified with a putative role in osk mRNA and protein anchoring [4]. This communication demonstrates that mutations in the Drosophila moesin (Dmoe) gene that encodes another actin binding protein result in delocalization of osk mRNA and protein from the posterior subcortical region and, as a consequence, in failure of embryonic germ cell development. In Dmoe mutant oocytes, the subcortical actin network is detached from the cell membrane, while the polarized microtubule cytoskeleton is unaffected. In line with the earlier observations [6, 8], colocalization of ectopic actin and OSK protein in Dmoe mutants suggests that the actin cytoskeleton anchors OSK protein to the subcortical cytoplasmic area of the Drosophila oocyte.

UR - http://www.scopus.com/inward/record.url?scp=0036898831&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036898831&partnerID=8YFLogxK

U2 - 10.1016/S0960-9822(02)01256-3

DO - 10.1016/S0960-9822(02)01256-3

M3 - Article

C2 - 12477397

AN - SCOPUS:0036898831

VL - 12

SP - 2060

EP - 2065

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 23

ER -