Following 35 min of adhesion to a plastic surface, an 80-kDa F-actin-binding protein was shown to be enriched in the plasma membrane fractions of porcine neutrophils by protein blotting with labeled F-actin. This protein was almost undetectable in membrane fractions of free floating neutrophils, while it was present in total cell samples. The 80-kDa protein appeared to be a major high molecular mass component of the isolated actin-cytoskeleton of both control and attached cells. The studied F-actin-binding protein was recognized by anti-moesin antibodies. Our results suggest that moesin is translocated to the plasma membrane upon adhesion of neutrophils to the extracellular surface.
|Number of pages||5|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - Nov 27 1998|
- Plasma membrane
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology