Modulative effect of endogenous granuloid inhibitors on cell proliferation

A. Balázs, T. Klupp, G. Zsila, I. Blazsek, L. Holczinger, D. Gaál

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The particle-free crude extract of differentiated granulocytes (GCE) and the GI-1, GI-2, GI-3 proliferation inhibitory fractions (M.W. ≥ 70,000 ∼ 11,500 and ≤ 4000) were studied for the effects they exert on cultured cells. As shown by the curve, in bone marrow suspension cultures, in the dose range of 0.01-10.0 μg/ml, GCE maximally inhibits 3H-TdR incorporation into the DNA for 5.5 hours. In agar-gel cultures, 1.0-5.0 μg/ml of GCE reduces both the number (by 50.6-54.8%) and the size of the colonies formed. The manifestation of the inhibitory effect of GI-1, GI-2 and GI-3 fractions requires 2.5-3.5 hours. The inhibitors designated GI-2 and GI-3 are target-cell specific but they do not reduce 3H-TdR incorporation either in thymocyte suspension or HeLa monolayer cultures. On the other hand GI-1 inhibits the proliferation in HeLa cultures, as well. These endogenous inhibitors exert their optimum effect, even in a partially purified state at 2-4 orders of magnitude lower concentrations than does 1, 2 5,6-dianhydrogalactitol (DAD), a cell-aspecific inhibitor used for comparison in the same system.

Original languageEnglish
Pages (from-to)207-218
Number of pages12
JournalMechanisms of Ageing and Development
Volume6
Issue numberC
DOIs
Publication statusPublished - 1977

Fingerprint

Cell proliferation
Granulocytes
Dianhydrogalactitol
Suspensions
Cell Proliferation
Complex Mixtures
Agar
Monolayers
Bone
Gels
Cells
Thymocytes
Cultured Cells
DNA
Bone Marrow

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Modulative effect of endogenous granuloid inhibitors on cell proliferation. / Balázs, A.; Klupp, T.; Zsila, G.; Blazsek, I.; Holczinger, L.; Gaál, D.

In: Mechanisms of Ageing and Development, Vol. 6, No. C, 1977, p. 207-218.

Research output: Contribution to journalArticle

Balázs, A. ; Klupp, T. ; Zsila, G. ; Blazsek, I. ; Holczinger, L. ; Gaál, D. / Modulative effect of endogenous granuloid inhibitors on cell proliferation. In: Mechanisms of Ageing and Development. 1977 ; Vol. 6, No. C. pp. 207-218.
@article{8d7fc1f42d7d43e9b3b430cbdc70b99e,
title = "Modulative effect of endogenous granuloid inhibitors on cell proliferation",
abstract = "The particle-free crude extract of differentiated granulocytes (GCE) and the GI-1, GI-2, GI-3 proliferation inhibitory fractions (M.W. ≥ 70,000 ∼ 11,500 and ≤ 4000) were studied for the effects they exert on cultured cells. As shown by the curve, in bone marrow suspension cultures, in the dose range of 0.01-10.0 μg/ml, GCE maximally inhibits 3H-TdR incorporation into the DNA for 5.5 hours. In agar-gel cultures, 1.0-5.0 μg/ml of GCE reduces both the number (by 50.6-54.8{\%}) and the size of the colonies formed. The manifestation of the inhibitory effect of GI-1, GI-2 and GI-3 fractions requires 2.5-3.5 hours. The inhibitors designated GI-2 and GI-3 are target-cell specific but they do not reduce 3H-TdR incorporation either in thymocyte suspension or HeLa monolayer cultures. On the other hand GI-1 inhibits the proliferation in HeLa cultures, as well. These endogenous inhibitors exert their optimum effect, even in a partially purified state at 2-4 orders of magnitude lower concentrations than does 1, 2 5,6-dianhydrogalactitol (DAD), a cell-aspecific inhibitor used for comparison in the same system.",
author = "A. Bal{\'a}zs and T. Klupp and G. Zsila and I. Blazsek and L. Holczinger and D. Ga{\'a}l",
year = "1977",
doi = "10.1016/0047-6374(77)90022-7",
language = "English",
volume = "6",
pages = "207--218",
journal = "Mechanisms of Ageing and Development",
issn = "0047-6374",
publisher = "Elsevier Ireland Ltd",
number = "C",

}

TY - JOUR

T1 - Modulative effect of endogenous granuloid inhibitors on cell proliferation

AU - Balázs, A.

AU - Klupp, T.

AU - Zsila, G.

AU - Blazsek, I.

AU - Holczinger, L.

AU - Gaál, D.

PY - 1977

Y1 - 1977

N2 - The particle-free crude extract of differentiated granulocytes (GCE) and the GI-1, GI-2, GI-3 proliferation inhibitory fractions (M.W. ≥ 70,000 ∼ 11,500 and ≤ 4000) were studied for the effects they exert on cultured cells. As shown by the curve, in bone marrow suspension cultures, in the dose range of 0.01-10.0 μg/ml, GCE maximally inhibits 3H-TdR incorporation into the DNA for 5.5 hours. In agar-gel cultures, 1.0-5.0 μg/ml of GCE reduces both the number (by 50.6-54.8%) and the size of the colonies formed. The manifestation of the inhibitory effect of GI-1, GI-2 and GI-3 fractions requires 2.5-3.5 hours. The inhibitors designated GI-2 and GI-3 are target-cell specific but they do not reduce 3H-TdR incorporation either in thymocyte suspension or HeLa monolayer cultures. On the other hand GI-1 inhibits the proliferation in HeLa cultures, as well. These endogenous inhibitors exert their optimum effect, even in a partially purified state at 2-4 orders of magnitude lower concentrations than does 1, 2 5,6-dianhydrogalactitol (DAD), a cell-aspecific inhibitor used for comparison in the same system.

AB - The particle-free crude extract of differentiated granulocytes (GCE) and the GI-1, GI-2, GI-3 proliferation inhibitory fractions (M.W. ≥ 70,000 ∼ 11,500 and ≤ 4000) were studied for the effects they exert on cultured cells. As shown by the curve, in bone marrow suspension cultures, in the dose range of 0.01-10.0 μg/ml, GCE maximally inhibits 3H-TdR incorporation into the DNA for 5.5 hours. In agar-gel cultures, 1.0-5.0 μg/ml of GCE reduces both the number (by 50.6-54.8%) and the size of the colonies formed. The manifestation of the inhibitory effect of GI-1, GI-2 and GI-3 fractions requires 2.5-3.5 hours. The inhibitors designated GI-2 and GI-3 are target-cell specific but they do not reduce 3H-TdR incorporation either in thymocyte suspension or HeLa monolayer cultures. On the other hand GI-1 inhibits the proliferation in HeLa cultures, as well. These endogenous inhibitors exert their optimum effect, even in a partially purified state at 2-4 orders of magnitude lower concentrations than does 1, 2 5,6-dianhydrogalactitol (DAD), a cell-aspecific inhibitor used for comparison in the same system.

UR - http://www.scopus.com/inward/record.url?scp=0017364639&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017364639&partnerID=8YFLogxK

U2 - 10.1016/0047-6374(77)90022-7

DO - 10.1016/0047-6374(77)90022-7

M3 - Article

C2 - 559224

AN - SCOPUS:0017364639

VL - 6

SP - 207

EP - 218

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

IS - C

ER -