Modulation of the truncated GAD25 by estrogen in the olfactory bulb of adult rats

István A. Krizbai, Zoya Katarova, Gábor Szabó, Árpád Párducz, Joachim R. Wolff

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The aim of our study was to investigate the influence of gonadal steroids-on the expression of different GAD isoforms. Here we show that, in addition to the adult GAD forms, the two embryonic splice variants of GAD67 mRNA and the truncated GAD25 are present in the adult rat olfactory bulb, a brain region with high synaptic plasticity, which has preserved some features of the developing brain. By Western blot analysis, we could demonstrate that the expression of the embryonic GAD25 is cyclic in females: its quantity is higher on estrus day. Furthermore in ovariectomized animals 17-β-estradiol treatment induced an increase of GAD25 within 3 h, reaching a maximum at 9-12 h. Our data are compatible with the interpretation that the embryonic GAD isoforms may play a role in the neuroplastic changes induced by sexual steroids. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)791-794
Number of pages4
JournalNeuroreport
Volume11
Issue number4
DOIs
Publication statusPublished - Mar 20 2000

Keywords

  • Embryonic GAD isoforms
  • Estrogen
  • Olfactory bulb
  • RT-PCR
  • Western blot

ASJC Scopus subject areas

  • Neuroscience(all)

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