Modulation of the humoral immune response by targeting CD40 and FcγRII/III; delivery of soluble but not particulate antigen to CD40 enhances antibody responses with a Th1 bias

Zsuzsanna Szekeres, Melinda Herbáth, Zoltán Szittner, Krisztián Papp, A. Erdei, J. Prechl

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Targeted delivery of antigen improves immunogenicity and can obviate the use of adjuvants. In addition to molecular targeting based on affinity interactions, particle-based antigen targeting to myeloid cells is also an efficient means to enhance immune responses. We compared the efficiency of targeting a model antigen, streptavidin, to CD40 and low affinity Fc gamma receptors II and III, either in a soluble or in a particulate form. Single chain fragments targeting these receptors were used to generate soluble tetramers with streptavidin or to decorate streptavidin coated nanobeads, and mice were immunized with the different formulations. Whereas particulate presentation of streptavidin enhanced total IgG1 and IgG2a levels, overall antigen specific antibody production increased in the case of targeted soluble antigen only, as assessed by reverse protein arrays and ELISPOT. In particular, soluble CD40 targeted antigen induced the strongest IgG2a responses, suggesting a Th1 bias compared to FcgammaRII/III targeting. Combined targeting to these receptors did not further increase immunogenicity.Thus, in our model, affinity targeting of soluble antigen to CD40 proved to be superior to particle-mediated delivery both in terms of antibody quantity and quality.

Original languageEnglish
Pages (from-to)155-162
Number of pages8
JournalMolecular Immunology
Volume49
Issue number1-2
DOIs
Publication statusPublished - Oct 2011

Fingerprint

CD40 Antigens
Humoral Immunity
Streptavidin
Antibody Formation
Antigens
Enzyme-Linked Immunospot Assay
IgG Receptors
Protein Array Analysis
Myeloid Cells
Immunoglobulin G
Antibodies

Keywords

  • Antigen
  • CD40
  • FcγRII/III
  • Nanoparticles
  • ScFv
  • Streptavidin
  • Targeting

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Modulation of the humoral immune response by targeting CD40 and FcγRII/III; delivery of soluble but not particulate antigen to CD40 enhances antibody responses with a Th1 bias. / Szekeres, Zsuzsanna; Herbáth, Melinda; Szittner, Zoltán; Papp, Krisztián; Erdei, A.; Prechl, J.

In: Molecular Immunology, Vol. 49, No. 1-2, 10.2011, p. 155-162.

Research output: Contribution to journalArticle

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