Modulation of recombinant Antigenic constructs containing multi-epitopes towards effective reduction of atherosclerotic lesion in B6;129S-Ldlrtm1HerApobtm2Sgy/J mice

Min Xia, Daxin Chen, V. Endrész, Ildiko Lantos, Andrea Szabo, Vijay Kakkar, Xinjie Lu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Atherosclerosis is increasingly recognized as a complex chronic inflammatory disease. Many more studies have extended vaccination against atherosclerosis by using epitopes from self-antigens or beyond and demonstrated that vaccination with antigens or derivatives could reduce the extent of the lesions in atherosclerosis-prone mice. Our previous study has demonstrated that construct AHHC [ApoB100688-707 + hHSP60303-312 + hHSP60153-163 + Cpn derived peptide (C)] significantly reduced atherosclerotic lesion. The aim of this study was to investigate whether AHHC can be modulated towards increased lesion reduction in mice by creating two other derivatives with a sequential epitope-substitution named RHHC in which A was replaced by an "R" (C5aR1-31) and RPHC with a further "H" (hHSP60303-312) conversion into "P" (protease-activated receptor-142-55) in mice. Antigenic epitopes were incorporated into a dendroaspin scaffold. Immunization of B6;129S-Ldlrtm1HerApobtm2Sgy/J mice with three constructs elicited production of high levels of antibodies against each epitope (apart from hHSP60153-163 and P which induced a low antibody response). Histological analyses demonstrated that the mice immunized with either RPHC or RHHC showed significant reductions in the size of atherosclerostic lesions compared to those with AHHC (69.5±1.1% versus 55.7±3.4%, P

Original languageEnglish
Article numbere0123393
JournalPLoS One
Volume10
Issue number4
DOIs
Publication statusPublished - Apr 1 2015

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Antigenic Modulation
epitopes
Epitopes
Modulation
atherosclerosis
mice
Atherosclerosis
Proteinase-Activated Receptors
Derivatives
Immunization
Vaccination
vaccination
Antibodies
Autoantigens
antigens
Scaffolds
antibodies
Substitution reactions
Antibody Formation
immunization

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Modulation of recombinant Antigenic constructs containing multi-epitopes towards effective reduction of atherosclerotic lesion in B6;129S-Ldlrtm1HerApobtm2Sgy/J mice. / Xia, Min; Chen, Daxin; Endrész, V.; Lantos, Ildiko; Szabo, Andrea; Kakkar, Vijay; Lu, Xinjie.

In: PLoS One, Vol. 10, No. 4, e0123393, 01.04.2015.

Research output: Contribution to journalArticle

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