Modulation of membrane fluidity in living protoplasts of Nicotiana plumbaginifolia by catalytic hydrogenation

László VIGH, Ferenc JOÓ, Ágnes CSÉPLÓ

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Abstract

A homogeneous water‐soluble Ru catalyst, has been incorporated into mesophyll protoplasts isolated from Nicotiana plumbaginifolia leaves. In the presence of hydrogen gas this complex causes an extensive loss of unsaturated fatty acid bonds and a concomitant increase in microviscosity of the cellular membranes. Although the gradual reduction of the level of unsaturation, per se, is accompanied by considerable cell damage, there is an optimum reaction time where approximately 50% of the protoplasts are still living and about 20% of the double bounds initially present in fatty acyl residues have undergone hydrogenation. The possible mechanism of the self regulatory process competing with the hydrogenation in the early stages of the reaction is also discussed.

Original languageEnglish
Pages (from-to)241-244
Number of pages4
JournalEuropean Journal of Biochemistry
Volume146
Issue number2
DOIs
Publication statusPublished - Jan 1985

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ASJC Scopus subject areas

  • Biochemistry

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