Modulation of lipid unsaturation and membrane fluid state in mammalian cells by stable transformation with the Δ9-desaturase gene of Saccharomyces cerevisiae

Zs Gyorfy, I. Horváth, G. Balogh, Á Domonkos, E. Duda, B. Maresca, L. Vígh

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The composition and physical state of membrane lipids determine the dynamic nature of membranes, which in turn, could directly be linked to the activity of various membrane-associated cellular functions. To better understand the molecular basis of different membrane-related phenomena we established a novel strategy to alter unsaturation of mammalian cell membranes with an identical genetic background. We transfected L929 mouse fibroblastoid cells with DNA constructs containing the Δ9-fatty acid desaturase gene (Ole1) of S. cerevisiae under the control of desaturase promoters derived either from wild type or mutant strains of the dimorphic fungus H. capsulatum.

Original languageEnglish
Pages (from-to)362-366
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume237
Issue number2
DOIs
Publication statusPublished - Aug 18 1997

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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