Modulation of Glucocorticosteroid Binding in Human Lymphoid, Monocytoid and Hepatoma Cell Lines by Inflammatory Cytokines Interleukin (IL)‐lβ, IL‐6 and Tumour Necrosis Factor (TNF)‐α

E. RAKASZ, A. GAL, J. BIRO, G. BALAS, A. FALUS

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35 Citations (Scopus)

Abstract

In order to elucidate the role of the inflammatory cytokines in regulating glucocorticosteroid binding (GCSB) and glucocorticosteroid receptor (GR) level we incubated a B‐cell line (CESS), a promonocytic cell line (U937) and a hepatoma cell Iine(HepG2)in the presence of varying concentrations of IL‐1β, IL‐6 and TNF‐α(for 24 h. Glucocorticosteroid binding was determined by the method of whole cell uptake', and the cellular appearance of the glucocorticosteroid receptor was detected by immunocytochemistry, A rise in the glucocorticosteroid binding was induced by all three cytokines. The increase in level of glucocorticosteroid receptors in the cells shown by immunocytochemistry was much more pronounced. However, antagonistic effects were demonstrated by both methods between IL‐6 and TNF‐α. and between IL‐1β and TNF‐α when they were applied simultaneously, in U937. Present data suggest that local imbalance in the ratio of these three cytokines in different pathological cases might influence the glucocorticosteroid sensitivity of the lymphocytes, monocytes and hepatocytes as target cells.

Original languageEnglish
Pages (from-to)684-689
Number of pages6
JournalScandinavian Journal of Immunology
Volume37
Issue number6
DOIs
Publication statusPublished - Jun 1993

ASJC Scopus subject areas

  • Immunology

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