Modulation of Fc receptors on human peripheral blood lymphocytes by antisera against β2 microglobulin, Ia or immunoglobulin

G. Sármay, A. Sanderson, J. Ivanyi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The association bewteen Fc receptors and other surface molecules was examined by EA-rosette (EAR) inhibition experiments. Twenty to 30% EAR were detected in suspensions of peripheral blood lymphocytes from normal individuals. Anti-β2 microglobulin (βMi) sera fully suppressed EAR whereas anti-Ig, anti-Ia sera and heat aggregated IgG inhibited only 50-60% EAR. Thus almost half of the detected EAR were apparently not surface Ig positive B cells. Rabbit and monkey anti-βMi sera suppressed EAR effectively whereas rat and chicken antisera, despite strong βMi binding capacity, inhibited EAR poorly. The latter result was ascribed on the basis of immunofluorescent analysis to inadequate capping of βMi. Incubation of PBL with whole antisera suppressed EAR to a similar degree at either 0° or 37°, whereas F(ab')2 fragments were inhibitory only at 37°. Taken together the results suggest that Fc receptors can be inhibited by antibodies with specificity against any cell surface antigen. The blocking mechanism may be due to steric hindrance by the Fc part of antibody molecules and/or F(ab')2 fragment mediating co-capping.

Original languageEnglish
Pages (from-to)339-345
Number of pages7
JournalImmunology
Volume36
Issue number2
Publication statusPublished - 1979

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Fc Receptors
Immunoglobulins
Immune Sera
Lymphocytes
Serum
Antibody Specificity
Surface Antigens
Haplorhini
Chickens
Suspensions
B-Lymphocytes
Immunoglobulin G
Hot Temperature
Rabbits
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Modulation of Fc receptors on human peripheral blood lymphocytes by antisera against β2 microglobulin, Ia or immunoglobulin. / Sármay, G.; Sanderson, A.; Ivanyi, J.

In: Immunology, Vol. 36, No. 2, 1979, p. 339-345.

Research output: Contribution to journalArticle

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