Modulation of endoplasmic reticulum calcium pump expression during T lymphocyte activation

Sophie Launay, Régis Bobe, Christine Lacabaratz-Porret, Raymonde Bredoux, Tünde Kovàcs, Jocelyne Enouf, Béla Papp

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Calcium mobilization from intracellular storage or ganelles is a key component of the second messenger system inducing cell activation. Calcium transport ATPases associated with intracellular calcium storage organelles play a major role in controlling this process by accumulating calcium from the cytosol into intracellular calcium pools. In this study the modulation of the expression of the sarco-endoplasmic reticulum calcium transport ATPase (SERCA) isoenzymes has been studied in lymphocytes undergoing phorbol myristate acetate and ionomycin-induced activation. In several T lymphocyte cell lines a combined treatment by the two drugs resulted in an approximately 90% decrease of the expression of the calcium pump isoform recognized by the PLIM430 isoform-specific antibody, whereas the expression of the SERCA 2b isoform was increased approximately 2-fold. Phorbol ester or ionomycin applied separately was ineffective. In Jurkat T cells the down modulation of expression of the SERCA isoform recognized by the PLIM430 antibody appeared concern instantly with the induction of interleukin-2 expression and could be inhibited by the immunosuppressant drug cyclosporine-A. These data indicate that T cell activation induces a selective and cyclosporine-A-sensitive modulation of the expression of the SERCA calcium pump isoforms. This reflects a profound reorganization of the calcium homeostasis of T cells undergoing activation and may open new avenues in the understanding of the plasticity of the calcium homeostasis of differentiating cells and in the pharmacological modulation of lymphocyte function.

Original languageEnglish
Pages (from-to)10746-10750
Number of pages5
JournalJournal of Biological Chemistry
Volume272
Issue number16
DOIs
Publication statusPublished - Apr 18 1997

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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