Modulation of Cu2+ accumulation by (aminoethoxyvinyl)glycine and methylglyoxal bis(guanylhydrazone), the inhibitors of stress ethylene and polyamine synthesis in wheat genotypes

I. Tari, J. Csiszár, Katalin Gémes, Ágnes Szepesi

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4 Citations (Scopus)

Abstract

The effectiveness of Cu2+ accumulation was investigated in three wheat cultivars with different copper sensitivity (Triticum aestivum cv. GK Tiszatáj, GK Kata and GK Öthalom). Supraoptimal Cu2+ concentrations result in toxicity symptoms in the sensitive genotype and increase the production of the stress hormone, ethylene both in the leaves and root tissues of wheat seedlings. The sensitive cultivar, cv. Öthalom produced less ethylene than the tolerant genotypes (cvs Tiszatáj and Kata) in the roots whether the ethylene measurements were done over the 6-h period after Cu2+ exposure. Levels of 1-aminocyclopropane-1- carboxylic acid (ACC), the immediate precursor of ethylene, did not change characteristically during this period in either the tolerant or sensitive seedlings. The biosynthesis of ethylene has a common intermediate, S-adenosylmethionine (SAM) with the synthesis of the polyamine spermidine and 2-deoxymugineic acid, a Fe3+-solubilizing and transporting wheat phytosiderophore. These chelating substances also mediate the transport of different bivalent cations, such as Cu2+. The biosynthetic pathways of ethylene and polyamines, spermidine and spermine may compete for SAM with the phytosiderophore synthesis. Simultaneous inhibition of SAM decarboxylase by 0.5 M methylglyoxal bis(guanylhydrazone) (MGBG), and 1-aminocyclopropane-1- carboxylic acid synthase by 10 μM (2-aminoethoxy-vinyl)glycine (AVG), significantly increased the Cu2+ accumulation in root tissues of the wheat cultivars independently of their sensitivities. MGBG alone resulted in an enhanced copper content but AVG proved to be ineffective. This suggests that the amount of SAM allocated for polyamine formation may limit the phytosiderophore synthesis or spermidine (spermine) in itself may control the uptake of Cu 2+.

Original languageEnglish
Pages (from-to)989-996
Number of pages8
JournalCereal Research Communications
Volume34
Issue number2-3
DOIs
Publication statusPublished - 2006

Fingerprint

Mitoguazone
aminoethoxyvinylglycine
Polyamines
polyamines
Glycine
Triticum
spermidine
ethylene
phytosiderophores
Spermidine
Genotype
S-adenosylmethionine
S-Adenosylmethionine
wheat
synthesis
genotype
1-aminocyclopropane-1-carboxylic acid
glycine (amino acid)
Seedlings
cultivars

Keywords

  • (2-aminoethoxyvinyl)glycine
  • Copper accumulation
  • Ethylene production
  • Methylglyoxal bis(guanylhydrazone)
  • Phytosiderophores
  • Spermidine synthesis

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

@article{5c8c378191dd4d068ef8b72a54801987,
title = "Modulation of Cu2+ accumulation by (aminoethoxyvinyl)glycine and methylglyoxal bis(guanylhydrazone), the inhibitors of stress ethylene and polyamine synthesis in wheat genotypes",
abstract = "The effectiveness of Cu2+ accumulation was investigated in three wheat cultivars with different copper sensitivity (Triticum aestivum cv. GK Tiszat{\'a}j, GK Kata and GK {\"O}thalom). Supraoptimal Cu2+ concentrations result in toxicity symptoms in the sensitive genotype and increase the production of the stress hormone, ethylene both in the leaves and root tissues of wheat seedlings. The sensitive cultivar, cv. {\"O}thalom produced less ethylene than the tolerant genotypes (cvs Tiszat{\'a}j and Kata) in the roots whether the ethylene measurements were done over the 6-h period after Cu2+ exposure. Levels of 1-aminocyclopropane-1- carboxylic acid (ACC), the immediate precursor of ethylene, did not change characteristically during this period in either the tolerant or sensitive seedlings. The biosynthesis of ethylene has a common intermediate, S-adenosylmethionine (SAM) with the synthesis of the polyamine spermidine and 2-deoxymugineic acid, a Fe3+-solubilizing and transporting wheat phytosiderophore. These chelating substances also mediate the transport of different bivalent cations, such as Cu2+. The biosynthetic pathways of ethylene and polyamines, spermidine and spermine may compete for SAM with the phytosiderophore synthesis. Simultaneous inhibition of SAM decarboxylase by 0.5 M methylglyoxal bis(guanylhydrazone) (MGBG), and 1-aminocyclopropane-1- carboxylic acid synthase by 10 μM (2-aminoethoxy-vinyl)glycine (AVG), significantly increased the Cu2+ accumulation in root tissues of the wheat cultivars independently of their sensitivities. MGBG alone resulted in an enhanced copper content but AVG proved to be ineffective. This suggests that the amount of SAM allocated for polyamine formation may limit the phytosiderophore synthesis or spermidine (spermine) in itself may control the uptake of Cu 2+.",
keywords = "(2-aminoethoxyvinyl)glycine, Copper accumulation, Ethylene production, Methylglyoxal bis(guanylhydrazone), Phytosiderophores, Spermidine synthesis",
author = "I. Tari and J. Csisz{\'a}r and Katalin G{\'e}mes and {\'A}gnes Szepesi",
year = "2006",
doi = "10.1556/CRC.34.2006.2-3.229",
language = "English",
volume = "34",
pages = "989--996",
journal = "Cereal Research Communications",
issn = "0133-3720",
publisher = "Cereal Research Non-Profit Company",
number = "2-3",

}

TY - JOUR

T1 - Modulation of Cu2+ accumulation by (aminoethoxyvinyl)glycine and methylglyoxal bis(guanylhydrazone), the inhibitors of stress ethylene and polyamine synthesis in wheat genotypes

AU - Tari, I.

AU - Csiszár, J.

AU - Gémes, Katalin

AU - Szepesi, Ágnes

PY - 2006

Y1 - 2006

N2 - The effectiveness of Cu2+ accumulation was investigated in three wheat cultivars with different copper sensitivity (Triticum aestivum cv. GK Tiszatáj, GK Kata and GK Öthalom). Supraoptimal Cu2+ concentrations result in toxicity symptoms in the sensitive genotype and increase the production of the stress hormone, ethylene both in the leaves and root tissues of wheat seedlings. The sensitive cultivar, cv. Öthalom produced less ethylene than the tolerant genotypes (cvs Tiszatáj and Kata) in the roots whether the ethylene measurements were done over the 6-h period after Cu2+ exposure. Levels of 1-aminocyclopropane-1- carboxylic acid (ACC), the immediate precursor of ethylene, did not change characteristically during this period in either the tolerant or sensitive seedlings. The biosynthesis of ethylene has a common intermediate, S-adenosylmethionine (SAM) with the synthesis of the polyamine spermidine and 2-deoxymugineic acid, a Fe3+-solubilizing and transporting wheat phytosiderophore. These chelating substances also mediate the transport of different bivalent cations, such as Cu2+. The biosynthetic pathways of ethylene and polyamines, spermidine and spermine may compete for SAM with the phytosiderophore synthesis. Simultaneous inhibition of SAM decarboxylase by 0.5 M methylglyoxal bis(guanylhydrazone) (MGBG), and 1-aminocyclopropane-1- carboxylic acid synthase by 10 μM (2-aminoethoxy-vinyl)glycine (AVG), significantly increased the Cu2+ accumulation in root tissues of the wheat cultivars independently of their sensitivities. MGBG alone resulted in an enhanced copper content but AVG proved to be ineffective. This suggests that the amount of SAM allocated for polyamine formation may limit the phytosiderophore synthesis or spermidine (spermine) in itself may control the uptake of Cu 2+.

AB - The effectiveness of Cu2+ accumulation was investigated in three wheat cultivars with different copper sensitivity (Triticum aestivum cv. GK Tiszatáj, GK Kata and GK Öthalom). Supraoptimal Cu2+ concentrations result in toxicity symptoms in the sensitive genotype and increase the production of the stress hormone, ethylene both in the leaves and root tissues of wheat seedlings. The sensitive cultivar, cv. Öthalom produced less ethylene than the tolerant genotypes (cvs Tiszatáj and Kata) in the roots whether the ethylene measurements were done over the 6-h period after Cu2+ exposure. Levels of 1-aminocyclopropane-1- carboxylic acid (ACC), the immediate precursor of ethylene, did not change characteristically during this period in either the tolerant or sensitive seedlings. The biosynthesis of ethylene has a common intermediate, S-adenosylmethionine (SAM) with the synthesis of the polyamine spermidine and 2-deoxymugineic acid, a Fe3+-solubilizing and transporting wheat phytosiderophore. These chelating substances also mediate the transport of different bivalent cations, such as Cu2+. The biosynthetic pathways of ethylene and polyamines, spermidine and spermine may compete for SAM with the phytosiderophore synthesis. Simultaneous inhibition of SAM decarboxylase by 0.5 M methylglyoxal bis(guanylhydrazone) (MGBG), and 1-aminocyclopropane-1- carboxylic acid synthase by 10 μM (2-aminoethoxy-vinyl)glycine (AVG), significantly increased the Cu2+ accumulation in root tissues of the wheat cultivars independently of their sensitivities. MGBG alone resulted in an enhanced copper content but AVG proved to be ineffective. This suggests that the amount of SAM allocated for polyamine formation may limit the phytosiderophore synthesis or spermidine (spermine) in itself may control the uptake of Cu 2+.

KW - (2-aminoethoxyvinyl)glycine

KW - Copper accumulation

KW - Ethylene production

KW - Methylglyoxal bis(guanylhydrazone)

KW - Phytosiderophores

KW - Spermidine synthesis

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U2 - 10.1556/CRC.34.2006.2-3.229

DO - 10.1556/CRC.34.2006.2-3.229

M3 - Article

AN - SCOPUS:33749517543

VL - 34

SP - 989

EP - 996

JO - Cereal Research Communications

JF - Cereal Research Communications

SN - 0133-3720

IS - 2-3

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