Modulation of chloroplast membrane lipids by homogeneous catalytic hydrogenation.

L. Vígh, F. Joó, M. Droppa, G. Horváth, G. Horváth

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A method is reported for the modification of lipids in situ in chloroplast membrane by which a homogeneous, water-soluble catalyst Pd(QS)2 (QS, sulphonated alizarine; C14H6O7NaS) is incorporated into the thylakoids of isolated chloroplast. The catalyst itself did not affect the photosynthetic activity but caused an extensive loss of unsaturated fatty acids in the presence of hydrogen gas. The polyunsaturated fatty acids were hydrogenated at a faster rate than the monoenoic acids. During hydrogenation the orientational ordering of membrane lipids, as measured with the C-12 positional isomer of spin-labelled stearic acid, displayed a slight increase in agreement with the alterations in membrane composition. Progressive saturation of double bonds of lipids primarily inhibits electron transport between the photosystems followed by the inhibition of electron flow around photosystem II. Photosystem I electron transport was not inhibited even by 50% fatty acid hydrogenation. We suggest that using Pd(QS)2 catalyst for thylakoid hydrogenation offers an excellent technique to study the role of various unsaturated fatty acids in the regulation of membrane fluidity and photosynthetic processes.

Original languageEnglish
Pages (from-to)477-481
Number of pages5
JournalEuropean Journal of Biochemistry
Volume147
Issue number3
Publication statusPublished - Mar 15 1985

Fingerprint

Hydrogenation
Chloroplasts
Membrane Lipids
Unsaturated Fatty Acids
Thylakoids
Modulation
Electron Transport
Membranes
Catalysts
Lipids
Photosystem I Protein Complex
Membrane Fluidity
Photosystem II Protein Complex
Fluidity
Isomers
Hydrogen
Fatty Acids
Gases
Electrons
Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Modulation of chloroplast membrane lipids by homogeneous catalytic hydrogenation. / Vígh, L.; Joó, F.; Droppa, M.; Horváth, G.; Horváth, G.

In: European Journal of Biochemistry, Vol. 147, No. 3, 15.03.1985, p. 477-481.

Research output: Contribution to journalArticle

@article{81601c5a9b704fd0a6115a23fd0cc053,
title = "Modulation of chloroplast membrane lipids by homogeneous catalytic hydrogenation.",
abstract = "A method is reported for the modification of lipids in situ in chloroplast membrane by which a homogeneous, water-soluble catalyst Pd(QS)2 (QS, sulphonated alizarine; C14H6O7NaS) is incorporated into the thylakoids of isolated chloroplast. The catalyst itself did not affect the photosynthetic activity but caused an extensive loss of unsaturated fatty acids in the presence of hydrogen gas. The polyunsaturated fatty acids were hydrogenated at a faster rate than the monoenoic acids. During hydrogenation the orientational ordering of membrane lipids, as measured with the C-12 positional isomer of spin-labelled stearic acid, displayed a slight increase in agreement with the alterations in membrane composition. Progressive saturation of double bonds of lipids primarily inhibits electron transport between the photosystems followed by the inhibition of electron flow around photosystem II. Photosystem I electron transport was not inhibited even by 50{\%} fatty acid hydrogenation. We suggest that using Pd(QS)2 catalyst for thylakoid hydrogenation offers an excellent technique to study the role of various unsaturated fatty acids in the regulation of membrane fluidity and photosynthetic processes.",
author = "L. V{\'i}gh and F. Jo{\'o} and M. Droppa and G. Horv{\'a}th and G. Horv{\'a}th",
year = "1985",
month = "3",
day = "15",
language = "English",
volume = "147",
pages = "477--481",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Modulation of chloroplast membrane lipids by homogeneous catalytic hydrogenation.

AU - Vígh, L.

AU - Joó, F.

AU - Droppa, M.

AU - Horváth, G.

AU - Horváth, G.

PY - 1985/3/15

Y1 - 1985/3/15

N2 - A method is reported for the modification of lipids in situ in chloroplast membrane by which a homogeneous, water-soluble catalyst Pd(QS)2 (QS, sulphonated alizarine; C14H6O7NaS) is incorporated into the thylakoids of isolated chloroplast. The catalyst itself did not affect the photosynthetic activity but caused an extensive loss of unsaturated fatty acids in the presence of hydrogen gas. The polyunsaturated fatty acids were hydrogenated at a faster rate than the monoenoic acids. During hydrogenation the orientational ordering of membrane lipids, as measured with the C-12 positional isomer of spin-labelled stearic acid, displayed a slight increase in agreement with the alterations in membrane composition. Progressive saturation of double bonds of lipids primarily inhibits electron transport between the photosystems followed by the inhibition of electron flow around photosystem II. Photosystem I electron transport was not inhibited even by 50% fatty acid hydrogenation. We suggest that using Pd(QS)2 catalyst for thylakoid hydrogenation offers an excellent technique to study the role of various unsaturated fatty acids in the regulation of membrane fluidity and photosynthetic processes.

AB - A method is reported for the modification of lipids in situ in chloroplast membrane by which a homogeneous, water-soluble catalyst Pd(QS)2 (QS, sulphonated alizarine; C14H6O7NaS) is incorporated into the thylakoids of isolated chloroplast. The catalyst itself did not affect the photosynthetic activity but caused an extensive loss of unsaturated fatty acids in the presence of hydrogen gas. The polyunsaturated fatty acids were hydrogenated at a faster rate than the monoenoic acids. During hydrogenation the orientational ordering of membrane lipids, as measured with the C-12 positional isomer of spin-labelled stearic acid, displayed a slight increase in agreement with the alterations in membrane composition. Progressive saturation of double bonds of lipids primarily inhibits electron transport between the photosystems followed by the inhibition of electron flow around photosystem II. Photosystem I electron transport was not inhibited even by 50% fatty acid hydrogenation. We suggest that using Pd(QS)2 catalyst for thylakoid hydrogenation offers an excellent technique to study the role of various unsaturated fatty acids in the regulation of membrane fluidity and photosynthetic processes.

UR - http://www.scopus.com/inward/record.url?scp=0022425666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022425666&partnerID=8YFLogxK

M3 - Article

C2 - 3979383

AN - SCOPUS:0022425666

VL - 147

SP - 477

EP - 481

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 3

ER -