Modulation of cell-cell and cell-antigen interactions by 1,25-dihydroxyvitamin D3 and vitamin D3 sulfate in vitro: a study on pregnancy lymphocytes and hybridoma cells

Joseph Najbauer, J. Szekeres-Barthó, Gábor J. Tigyi

Research output: Contribution to journalArticle

Abstract

The effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3 was investigated in single cell cytotoxicity assays, using K-562 target cells. The action of vitamin D3 sulfate (VD3S) in natural cytotoxicity assays as well as its effect on the antigen-specific adherence of hybridoma cells has also been studied. In the single cell cytotoxicity assay 1,25(OH2)D3 dose-dependently and significantly increased the binding of PBMC to target, the number of lysed target cells and NK activity, RU486, a compound known as a potent blocker of progesterone and glucocorticoid receptors, suppressed the effect of 1,25(OH2)D3 in all systems. VD3S dose-dependently decreased the natural cytotoxicity of PBMC and the binding of hybridoma cells to antigen immobilized on plastic surfaces. The results suggest that both 1,25(OH2)D3 and VD3S are potent.

Original languageEnglish
Pages (from-to)317-322
Number of pages6
JournalImmunology Letters
Volume20
Issue number4
DOIs
Publication statusPublished - 1989

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Calcitriol
Hybridomas
Cell Communication
Lymphocytes
Antigens
Pregnancy
Glucocorticoid Receptors
Progesterone Receptors
Natural Killer Cells
Plastics
In Vitro Techniques
vitamin D3 sulfoconjugate

Keywords

  • Hybridoma cell adherence
  • NK activity
  • Vitamin D

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

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title = "Modulation of cell-cell and cell-antigen interactions by 1,25-dihydroxyvitamin D3 and vitamin D3 sulfate in vitro: a study on pregnancy lymphocytes and hybridoma cells",
abstract = "The effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3 was investigated in single cell cytotoxicity assays, using K-562 target cells. The action of vitamin D3 sulfate (VD3S) in natural cytotoxicity assays as well as its effect on the antigen-specific adherence of hybridoma cells has also been studied. In the single cell cytotoxicity assay 1,25(OH2)D3 dose-dependently and significantly increased the binding of PBMC to target, the number of lysed target cells and NK activity, RU486, a compound known as a potent blocker of progesterone and glucocorticoid receptors, suppressed the effect of 1,25(OH2)D3 in all systems. VD3S dose-dependently decreased the natural cytotoxicity of PBMC and the binding of hybridoma cells to antigen immobilized on plastic surfaces. The results suggest that both 1,25(OH2)D3 and VD3S are potent.",
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T2 - a study on pregnancy lymphocytes and hybridoma cells

AU - Najbauer, Joseph

AU - Szekeres-Barthó, J.

AU - Tigyi, Gábor J.

PY - 1989

Y1 - 1989

N2 - The effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3 was investigated in single cell cytotoxicity assays, using K-562 target cells. The action of vitamin D3 sulfate (VD3S) in natural cytotoxicity assays as well as its effect on the antigen-specific adherence of hybridoma cells has also been studied. In the single cell cytotoxicity assay 1,25(OH2)D3 dose-dependently and significantly increased the binding of PBMC to target, the number of lysed target cells and NK activity, RU486, a compound known as a potent blocker of progesterone and glucocorticoid receptors, suppressed the effect of 1,25(OH2)D3 in all systems. VD3S dose-dependently decreased the natural cytotoxicity of PBMC and the binding of hybridoma cells to antigen immobilized on plastic surfaces. The results suggest that both 1,25(OH2)D3 and VD3S are potent.

AB - The effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3 was investigated in single cell cytotoxicity assays, using K-562 target cells. The action of vitamin D3 sulfate (VD3S) in natural cytotoxicity assays as well as its effect on the antigen-specific adherence of hybridoma cells has also been studied. In the single cell cytotoxicity assay 1,25(OH2)D3 dose-dependently and significantly increased the binding of PBMC to target, the number of lysed target cells and NK activity, RU486, a compound known as a potent blocker of progesterone and glucocorticoid receptors, suppressed the effect of 1,25(OH2)D3 in all systems. VD3S dose-dependently decreased the natural cytotoxicity of PBMC and the binding of hybridoma cells to antigen immobilized on plastic surfaces. The results suggest that both 1,25(OH2)D3 and VD3S are potent.

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KW - NK activity

KW - Vitamin D

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