Modified enzyme-linked immunosorbent assay for detecting enteroinvasive Escherichia coli and virulent Shigella strains

T. Pal, A. S. Pacsa, L. Emődy, S. Vörös, E. Sélley

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Immune sera were produced in rabbits with living cells of an enteroinvasive O143 strain of Escherichia coli. To remove O and K antibodies, sera were adsorbed with an avirulent derivative of the same strain. In the enzyme-linked immunosorbent assay, absorbed sera reacted specifically with only virulent Shigella strains and enteroinvasive E. coli strains of different geographical origin, regardless of species or serogroups. The investigation of 83 strains indicated complete agreement between enzyme-linked immunosorbent assay results and those of the keratoconjunctivitis test. It is assumed that the absorbed immune sera reacted with a possible virulence marker antigen. This inexpensive and simple method provides an alternative to other virulence tests. It has a definite advantage for screening large number of isolates within 24 h.

Original languageEnglish
Pages (from-to)415-418
Number of pages4
JournalJournal of Clinical Microbiology
Volume21
Issue number3
Publication statusPublished - 1985

Fingerprint

Shigella
Virulence
Immune Sera
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Keratoconjunctivitis
Differentiation Antigens
Serum
Rabbits
Antibodies
Serogroup

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Modified enzyme-linked immunosorbent assay for detecting enteroinvasive Escherichia coli and virulent Shigella strains. / Pal, T.; Pacsa, A. S.; Emődy, L.; Vörös, S.; Sélley, E.

In: Journal of Clinical Microbiology, Vol. 21, No. 3, 1985, p. 415-418.

Research output: Contribution to journalArticle

@article{d3de39c02f8b4840b5b3030f15f8a0ab,
title = "Modified enzyme-linked immunosorbent assay for detecting enteroinvasive Escherichia coli and virulent Shigella strains",
abstract = "Immune sera were produced in rabbits with living cells of an enteroinvasive O143 strain of Escherichia coli. To remove O and K antibodies, sera were adsorbed with an avirulent derivative of the same strain. In the enzyme-linked immunosorbent assay, absorbed sera reacted specifically with only virulent Shigella strains and enteroinvasive E. coli strains of different geographical origin, regardless of species or serogroups. The investigation of 83 strains indicated complete agreement between enzyme-linked immunosorbent assay results and those of the keratoconjunctivitis test. It is assumed that the absorbed immune sera reacted with a possible virulence marker antigen. This inexpensive and simple method provides an alternative to other virulence tests. It has a definite advantage for screening large number of isolates within 24 h.",
author = "T. Pal and Pacsa, {A. S.} and L. Emődy and S. V{\"o}r{\"o}s and E. S{\'e}lley",
year = "1985",
language = "English",
volume = "21",
pages = "415--418",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Modified enzyme-linked immunosorbent assay for detecting enteroinvasive Escherichia coli and virulent Shigella strains

AU - Pal, T.

AU - Pacsa, A. S.

AU - Emődy, L.

AU - Vörös, S.

AU - Sélley, E.

PY - 1985

Y1 - 1985

N2 - Immune sera were produced in rabbits with living cells of an enteroinvasive O143 strain of Escherichia coli. To remove O and K antibodies, sera were adsorbed with an avirulent derivative of the same strain. In the enzyme-linked immunosorbent assay, absorbed sera reacted specifically with only virulent Shigella strains and enteroinvasive E. coli strains of different geographical origin, regardless of species or serogroups. The investigation of 83 strains indicated complete agreement between enzyme-linked immunosorbent assay results and those of the keratoconjunctivitis test. It is assumed that the absorbed immune sera reacted with a possible virulence marker antigen. This inexpensive and simple method provides an alternative to other virulence tests. It has a definite advantage for screening large number of isolates within 24 h.

AB - Immune sera were produced in rabbits with living cells of an enteroinvasive O143 strain of Escherichia coli. To remove O and K antibodies, sera were adsorbed with an avirulent derivative of the same strain. In the enzyme-linked immunosorbent assay, absorbed sera reacted specifically with only virulent Shigella strains and enteroinvasive E. coli strains of different geographical origin, regardless of species or serogroups. The investigation of 83 strains indicated complete agreement between enzyme-linked immunosorbent assay results and those of the keratoconjunctivitis test. It is assumed that the absorbed immune sera reacted with a possible virulence marker antigen. This inexpensive and simple method provides an alternative to other virulence tests. It has a definite advantage for screening large number of isolates within 24 h.

UR - http://www.scopus.com/inward/record.url?scp=0021998343&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021998343&partnerID=8YFLogxK

M3 - Article

VL - 21

SP - 415

EP - 418

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

ER -