Moderate heat stress induces state transitions in Arabidopsis thaliana

Sreedhar Nellaepalli, Nageswara Rao Mekala, Otto Zsiros, Prasanna Mohanty, S. Rajagopal

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The effect of temperature on the photosynthetic machinery is crucial for the fundamental understanding of plant physiology and the bioengineering of heat-tolerant varieties. In our study, Arabidopsis thaliana was exposed to mild (40 °C), short-term heat stress in the dark to evaluate the heat-triggered phosphorylation and migration of light harvesting complex (LHC) II in both wild-type (wt) and mutant lacking STN7 kinase. The 77 K emission spectra revealed an increase in PSI relative to PSII emission similar to increases observed in light-induced state I to state II transitions in wt but not in stn7 mutant. Immunoblotting results indicated that the major LHCII was phosphorylated at threonine sites under heat stress in wt plants but not in the mutant. These results support the proposition that mild heat stress triggers state transitions in the dark similar to light-induced state transitions, which involve phosphorylation of LHCII by STN7 kinase. Pre-treatment of Arabidopsis leaves with inhibitor DBMIB, altered the extent of LHCII phosphorylation and PSI fluorescence emission suggests that activation of STN7 kinase may be dependent on Cyt b6/f under elevated temperatures in dark. Furthermore, fast Chl a transient of temperature-exposed leaves of wt showed a decrease in the Fv/Fm ratio due to both an increase in Fo and a decrease in Fm. In summary, our findings indicate that a mild heat treatment (40 °C) induces state transitions in the dark resulting in the migration of phosphorylated LHCII from the grana to the stroma region.

Original languageEnglish
Pages (from-to)1177-1184
Number of pages8
JournalBBA - Bioenergetics
Volume1807
Issue number9
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Arabidopsis
Hot Temperature
Phosphorylation
Phosphotransferases
Light
Temperature
Plant Physiological Phenomena
Physiology
Bioengineering
Threonine
Thylakoids
Machinery
Immunoblotting
Fluorescence
Chemical activation
Heat treatment

Keywords

  • Arabidopsis thaliana
  • Elevated temperature
  • Photosystems
  • PQ pool
  • State transition
  • STN7 kinase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Moderate heat stress induces state transitions in Arabidopsis thaliana. / Nellaepalli, Sreedhar; Mekala, Nageswara Rao; Zsiros, Otto; Mohanty, Prasanna; Rajagopal, S.

In: BBA - Bioenergetics, Vol. 1807, No. 9, 09.2011, p. 1177-1184.

Research output: Contribution to journalArticle

Nellaepalli, Sreedhar ; Mekala, Nageswara Rao ; Zsiros, Otto ; Mohanty, Prasanna ; Rajagopal, S. / Moderate heat stress induces state transitions in Arabidopsis thaliana. In: BBA - Bioenergetics. 2011 ; Vol. 1807, No. 9. pp. 1177-1184.
@article{eb81c1737c4f483eb10b0356de331811,
title = "Moderate heat stress induces state transitions in Arabidopsis thaliana",
abstract = "The effect of temperature on the photosynthetic machinery is crucial for the fundamental understanding of plant physiology and the bioengineering of heat-tolerant varieties. In our study, Arabidopsis thaliana was exposed to mild (40 °C), short-term heat stress in the dark to evaluate the heat-triggered phosphorylation and migration of light harvesting complex (LHC) II in both wild-type (wt) and mutant lacking STN7 kinase. The 77 K emission spectra revealed an increase in PSI relative to PSII emission similar to increases observed in light-induced state I to state II transitions in wt but not in stn7 mutant. Immunoblotting results indicated that the major LHCII was phosphorylated at threonine sites under heat stress in wt plants but not in the mutant. These results support the proposition that mild heat stress triggers state transitions in the dark similar to light-induced state transitions, which involve phosphorylation of LHCII by STN7 kinase. Pre-treatment of Arabidopsis leaves with inhibitor DBMIB, altered the extent of LHCII phosphorylation and PSI fluorescence emission suggests that activation of STN7 kinase may be dependent on Cyt b6/f under elevated temperatures in dark. Furthermore, fast Chl a transient of temperature-exposed leaves of wt showed a decrease in the Fv/Fm ratio due to both an increase in Fo and a decrease in Fm. In summary, our findings indicate that a mild heat treatment (40 °C) induces state transitions in the dark resulting in the migration of phosphorylated LHCII from the grana to the stroma region.",
keywords = "Arabidopsis thaliana, Elevated temperature, Photosystems, PQ pool, State transition, STN7 kinase",
author = "Sreedhar Nellaepalli and Mekala, {Nageswara Rao} and Otto Zsiros and Prasanna Mohanty and S. Rajagopal",
year = "2011",
month = "9",
doi = "10.1016/j.bbabio.2011.05.016",
language = "English",
volume = "1807",
pages = "1177--1184",
journal = "Biochimica et Biophysica Acta - Bioenergetics",
issn = "0005-2728",
publisher = "Elsevier",
number = "9",

}

TY - JOUR

T1 - Moderate heat stress induces state transitions in Arabidopsis thaliana

AU - Nellaepalli, Sreedhar

AU - Mekala, Nageswara Rao

AU - Zsiros, Otto

AU - Mohanty, Prasanna

AU - Rajagopal, S.

PY - 2011/9

Y1 - 2011/9

N2 - The effect of temperature on the photosynthetic machinery is crucial for the fundamental understanding of plant physiology and the bioengineering of heat-tolerant varieties. In our study, Arabidopsis thaliana was exposed to mild (40 °C), short-term heat stress in the dark to evaluate the heat-triggered phosphorylation and migration of light harvesting complex (LHC) II in both wild-type (wt) and mutant lacking STN7 kinase. The 77 K emission spectra revealed an increase in PSI relative to PSII emission similar to increases observed in light-induced state I to state II transitions in wt but not in stn7 mutant. Immunoblotting results indicated that the major LHCII was phosphorylated at threonine sites under heat stress in wt plants but not in the mutant. These results support the proposition that mild heat stress triggers state transitions in the dark similar to light-induced state transitions, which involve phosphorylation of LHCII by STN7 kinase. Pre-treatment of Arabidopsis leaves with inhibitor DBMIB, altered the extent of LHCII phosphorylation and PSI fluorescence emission suggests that activation of STN7 kinase may be dependent on Cyt b6/f under elevated temperatures in dark. Furthermore, fast Chl a transient of temperature-exposed leaves of wt showed a decrease in the Fv/Fm ratio due to both an increase in Fo and a decrease in Fm. In summary, our findings indicate that a mild heat treatment (40 °C) induces state transitions in the dark resulting in the migration of phosphorylated LHCII from the grana to the stroma region.

AB - The effect of temperature on the photosynthetic machinery is crucial for the fundamental understanding of plant physiology and the bioengineering of heat-tolerant varieties. In our study, Arabidopsis thaliana was exposed to mild (40 °C), short-term heat stress in the dark to evaluate the heat-triggered phosphorylation and migration of light harvesting complex (LHC) II in both wild-type (wt) and mutant lacking STN7 kinase. The 77 K emission spectra revealed an increase in PSI relative to PSII emission similar to increases observed in light-induced state I to state II transitions in wt but not in stn7 mutant. Immunoblotting results indicated that the major LHCII was phosphorylated at threonine sites under heat stress in wt plants but not in the mutant. These results support the proposition that mild heat stress triggers state transitions in the dark similar to light-induced state transitions, which involve phosphorylation of LHCII by STN7 kinase. Pre-treatment of Arabidopsis leaves with inhibitor DBMIB, altered the extent of LHCII phosphorylation and PSI fluorescence emission suggests that activation of STN7 kinase may be dependent on Cyt b6/f under elevated temperatures in dark. Furthermore, fast Chl a transient of temperature-exposed leaves of wt showed a decrease in the Fv/Fm ratio due to both an increase in Fo and a decrease in Fm. In summary, our findings indicate that a mild heat treatment (40 °C) induces state transitions in the dark resulting in the migration of phosphorylated LHCII from the grana to the stroma region.

KW - Arabidopsis thaliana

KW - Elevated temperature

KW - Photosystems

KW - PQ pool

KW - State transition

KW - STN7 kinase

UR - http://www.scopus.com/inward/record.url?scp=79960562176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960562176&partnerID=8YFLogxK

U2 - 10.1016/j.bbabio.2011.05.016

DO - 10.1016/j.bbabio.2011.05.016

M3 - Article

C2 - 21640068

AN - SCOPUS:79960562176

VL - 1807

SP - 1177

EP - 1184

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

SN - 0005-2728

IS - 9

ER -