Modeling the presentation of C3d-coated antigen by B lymphocytes: Enhancement by CR1/2-BCR co-ligation is selective for the co-ligating antigen

J. Prechl, Dana C. Baiu, Attila Horv́th, A. Erdei

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have used a set of single-chain variable fragment antibodies (sc) genetically fused with an influenza hemagglutinin-derived peptide as a means to investigate the role of CR1 and CR2 in antigen presentation by B cells. When incubated with the B cell lymphoma 2PK3, peptide-containing sc specific for either CR1 or CR1/2 mediated activation of the hemagglutinin peptide-specific T cell line IP-12-7, as assessed by IL-2 production. Efficient presentation was dependent on the binding of the constructs to CR1/2, implying that receptor-mediated endocytosis is responsible for the effect. Cross-linkage of CR1/2 or CD19 by mAb did not increase the extent of T cell activation. However, when CR1/2 was co-ligated with the BCR-using either polyclonal goat anti-mouse IgG or recombinant protein LA-the antigen concentration required to activate T cells decreased by two orders of magnitude. Moreover, this enhancement was selective for the antigen included in these complexes and did not affect the presentation of a free peptide or of antigen bound to CR1/2 excluded from the complexes. These results suggest that B cells may bind various C3d-coated antigens at a time, but only the one which reacts with the BCR will be processed with high efficiency. This mechanism may ensure the specificity of cognate T cell help.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalInternational Immunology
Volume14
Issue number3
Publication statusPublished - 2002

Fingerprint

Ligation
B-Lymphocytes
Hemagglutinins
T-Lymphocytes
Antigens
Peptides
T-Cell Antigen Receptor Specificity
Peptide T
Single-Chain Antibodies
Antigen Presentation
B-Cell Lymphoma
Endocytosis
Recombinant Proteins
Goats
Human Influenza
Interleukin-2
Cell Line
anti-IgG
SS-B antigen

Keywords

  • Antigen presentation
  • B lymphocytes
  • Complement

ASJC Scopus subject areas

  • Immunology

Cite this

Modeling the presentation of C3d-coated antigen by B lymphocytes : Enhancement by CR1/2-BCR co-ligation is selective for the co-ligating antigen. / Prechl, J.; Baiu, Dana C.; Horv́th, Attila; Erdei, A.

In: International Immunology, Vol. 14, No. 3, 2002, p. 241-247.

Research output: Contribution to journalArticle

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