Modeling of sensing potency of cytoskeletal systems decorated with metabolic enzymes

J. Oláh, Vic Norris, J. Ovádi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The highly dynamic cytoskeleton interacts with enzymes and other proteins that are involved in metabolic or signaling pathways. These interactions can influence the structural and functional characteristics of the partners at the microscopic level of individual proteins and polymers. In this work the functional consequences of such interactions have been studied at the macroscopic level in order to evaluate the integrative and regulatory roles of the metabolic pathways associated with the microtubule cytoskeleton. Here we present mathematical models of the interactions between a hypothetical metabolic pathway and microtubule assembly, and explore for the first time the functional consequences of these interactions in distinct situations. The models include kinetic constants of the individual steps and testable, relevant parameters which allow the quantification of the coupled processes at the microscopic and macroscopic levels. For example our kinetic model for the self-assembly of microtubules reproduces the alteration of the time-dependent turbidity caused by pyruvate kinase binding. Our data reveal the power of a mechanistic description of a filamentous system to explain how cells sense the state of metabolic and other pathways.

Original languageEnglish
Pages (from-to)190-196
Number of pages7
JournalJournal of Theoretical Biology
Volume365
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Metabolic Networks and Pathways
Microtubules
microtubules
Enzymes
Sensing
cytoskeleton
Cytoskeleton
Proteins
Pathway
Kinetics
Pyruvate Kinase
biochemical pathways
Turbidity
Kinetic Model
enzymes
Interaction
Modeling
Self assembly
kinetics
Polymers

Keywords

  • Mathematical model
  • Metabolism
  • Microtubule
  • Sensing

ASJC Scopus subject areas

  • Applied Mathematics
  • Statistics and Probability
  • Modelling and Simulation
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Modeling of sensing potency of cytoskeletal systems decorated with metabolic enzymes. / Oláh, J.; Norris, Vic; Ovádi, J.

In: Journal of Theoretical Biology, Vol. 365, 01.01.2015, p. 190-196.

Research output: Contribution to journalArticle

@article{07371ab3a4434c62bf5ac47576bd7b6d,
title = "Modeling of sensing potency of cytoskeletal systems decorated with metabolic enzymes",
abstract = "The highly dynamic cytoskeleton interacts with enzymes and other proteins that are involved in metabolic or signaling pathways. These interactions can influence the structural and functional characteristics of the partners at the microscopic level of individual proteins and polymers. In this work the functional consequences of such interactions have been studied at the macroscopic level in order to evaluate the integrative and regulatory roles of the metabolic pathways associated with the microtubule cytoskeleton. Here we present mathematical models of the interactions between a hypothetical metabolic pathway and microtubule assembly, and explore for the first time the functional consequences of these interactions in distinct situations. The models include kinetic constants of the individual steps and testable, relevant parameters which allow the quantification of the coupled processes at the microscopic and macroscopic levels. For example our kinetic model for the self-assembly of microtubules reproduces the alteration of the time-dependent turbidity caused by pyruvate kinase binding. Our data reveal the power of a mechanistic description of a filamentous system to explain how cells sense the state of metabolic and other pathways.",
keywords = "Mathematical model, Metabolism, Microtubule, Sensing",
author = "J. Ol{\'a}h and Vic Norris and J. Ov{\'a}di",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.jtbi.2014.10.018",
language = "English",
volume = "365",
pages = "190--196",
journal = "Journal of Theoretical Biology",
issn = "0022-5193",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Modeling of sensing potency of cytoskeletal systems decorated with metabolic enzymes

AU - Oláh, J.

AU - Norris, Vic

AU - Ovádi, J.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The highly dynamic cytoskeleton interacts with enzymes and other proteins that are involved in metabolic or signaling pathways. These interactions can influence the structural and functional characteristics of the partners at the microscopic level of individual proteins and polymers. In this work the functional consequences of such interactions have been studied at the macroscopic level in order to evaluate the integrative and regulatory roles of the metabolic pathways associated with the microtubule cytoskeleton. Here we present mathematical models of the interactions between a hypothetical metabolic pathway and microtubule assembly, and explore for the first time the functional consequences of these interactions in distinct situations. The models include kinetic constants of the individual steps and testable, relevant parameters which allow the quantification of the coupled processes at the microscopic and macroscopic levels. For example our kinetic model for the self-assembly of microtubules reproduces the alteration of the time-dependent turbidity caused by pyruvate kinase binding. Our data reveal the power of a mechanistic description of a filamentous system to explain how cells sense the state of metabolic and other pathways.

AB - The highly dynamic cytoskeleton interacts with enzymes and other proteins that are involved in metabolic or signaling pathways. These interactions can influence the structural and functional characteristics of the partners at the microscopic level of individual proteins and polymers. In this work the functional consequences of such interactions have been studied at the macroscopic level in order to evaluate the integrative and regulatory roles of the metabolic pathways associated with the microtubule cytoskeleton. Here we present mathematical models of the interactions between a hypothetical metabolic pathway and microtubule assembly, and explore for the first time the functional consequences of these interactions in distinct situations. The models include kinetic constants of the individual steps and testable, relevant parameters which allow the quantification of the coupled processes at the microscopic and macroscopic levels. For example our kinetic model for the self-assembly of microtubules reproduces the alteration of the time-dependent turbidity caused by pyruvate kinase binding. Our data reveal the power of a mechanistic description of a filamentous system to explain how cells sense the state of metabolic and other pathways.

KW - Mathematical model

KW - Metabolism

KW - Microtubule

KW - Sensing

UR - http://www.scopus.com/inward/record.url?scp=84911363736&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84911363736&partnerID=8YFLogxK

U2 - 10.1016/j.jtbi.2014.10.018

DO - 10.1016/j.jtbi.2014.10.018

M3 - Article

C2 - 25451961

AN - SCOPUS:84911363736

VL - 365

SP - 190

EP - 196

JO - Journal of Theoretical Biology

JF - Journal of Theoretical Biology

SN - 0022-5193

ER -