Modeling and biochemical analysis of the activity of antibiofilm agent Dispersin B

J. E. Kerrigan, C. Ragunath, L. Kandra, G. Gyémánt, A. Lipták, L. Jánossy, J. B. Kaplan, N. Ramasubbu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bacteria in a biofilm are enmeshed in a self-synthesized extracellular polysaccharide matrix (PGA), which is a linear polymer of β(1,6)-linked N-acetylglucosamine (GlcNAc) residues. Dispersin B (DspB), a soluble glycoside hydrolase produced by the periodontal pathogen Actinobacillus actinomycetemcomitans degrades PGA. The enzyme DspB is an α/β TIM-barrel protein and belongs to family 20 glycosyl hydrolases members. The enzyme activity of DspB with regard to its substrate specificity towards β(1,6)-linked GlcNAc polymers and its endo/exo character was investigated through ligand docking and the hydrolysis of synthetic oligosaccharides. Ligand docking analysis suggested that β(1,6)-linked GlcNAc oligosaccharide bound to the active site better that β(1,4)-linked GlcNAc oligosaccharide. Our combined results indicate that DspB is an exo-acting enzyme that hydrolyzes β(1,6)-linked N-acetylglucosamine oligomers.

Original languageEnglish
Pages (from-to)439-451
Number of pages13
JournalActa Biologica Hungarica
Volume59
Issue number4
DOIs
Publication statusPublished - Dec 2008

Fingerprint

Oligosaccharides
Prostaglandins A
ligand
Acetylglucosamine
polymer
enzyme
Polymers
Enzymes
polysaccharide
Ligands
modeling
enzyme activity
biofilm
Aggregatibacter actinomycetemcomitans
hydrolysis
pathogen
Glycoside Hydrolases
Biofilms
Enzyme activity
Hydrolases

Keywords

  • Biofilm
  • Dispersin B
  • Exo-acting
  • HPLC
  • Hydrolysis
  • Molecular modeling

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Modeling and biochemical analysis of the activity of antibiofilm agent Dispersin B. / Kerrigan, J. E.; Ragunath, C.; Kandra, L.; Gyémánt, G.; Lipták, A.; Jánossy, L.; Kaplan, J. B.; Ramasubbu, N.

In: Acta Biologica Hungarica, Vol. 59, No. 4, 12.2008, p. 439-451.

Research output: Contribution to journalArticle

Kerrigan, J. E. ; Ragunath, C. ; Kandra, L. ; Gyémánt, G. ; Lipták, A. ; Jánossy, L. ; Kaplan, J. B. ; Ramasubbu, N. / Modeling and biochemical analysis of the activity of antibiofilm agent Dispersin B. In: Acta Biologica Hungarica. 2008 ; Vol. 59, No. 4. pp. 439-451.
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