MMP activity detection in zymograms

Péter Bencsik, Monika Bartekova, A. Görbe, Krisztina Kiss, János Pálóczi, Jana Radosinska, Gergő Szűcs, Péter Ferdinandy

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Matrix metalloproteinases (MMP) belong to a distinguished class of zinc-dependent endopeptidases. Zymography is a semi-quantitative tool for determining the activity of different MMP isoenzymes in a variety of biological samples. In substrate gel zymography, protein samples of different origin (tissue, cell lysates, plasma/serum, perfusates, other liquids) are separated in sodium dodecyl sulfate (SDS) polyacrylamide gels containing copolymerized substrate (gelatin, casein, elastin, etc.), and after incubation-enabling substrate cleavage by MMPs, MMP activities are detected after the gel staining as transparent bands against a dark-blue background. In situ zymography is a histological modification of substrate zymography in frozen sections, allowing detection of the localization of the MMP activities within the tissue. Here, we describe detailed experimental protocols of all abovementioned techniques and provide examples for several sample measurements.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages53-70
Number of pages18
DOIs
Publication statusPublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1626
ISSN (Print)1064-3745

Fingerprint

Matrix Metalloproteinases
Gels
Endopeptidases
Elastin
Frozen Sections
Gelatin
Caseins
Plasma Cells
Sodium Dodecyl Sulfate
Isoenzymes
Zinc
Staining and Labeling
Serum
Proteins

Keywords

  • Casein
  • Gelatin
  • In situ zymography
  • Matrix metalloproteinase activity
  • Substrate zymography

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bencsik, P., Bartekova, M., Görbe, A., Kiss, K., Pálóczi, J., Radosinska, J., ... Ferdinandy, P. (2017). MMP activity detection in zymograms. In Methods in Molecular Biology (pp. 53-70). (Methods in Molecular Biology; Vol. 1626). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7111-4_6

MMP activity detection in zymograms. / Bencsik, Péter; Bartekova, Monika; Görbe, A.; Kiss, Krisztina; Pálóczi, János; Radosinska, Jana; Szűcs, Gergő; Ferdinandy, Péter.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 53-70 (Methods in Molecular Biology; Vol. 1626).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bencsik, P, Bartekova, M, Görbe, A, Kiss, K, Pálóczi, J, Radosinska, J, Szűcs, G & Ferdinandy, P 2017, MMP activity detection in zymograms. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1626, Humana Press Inc., pp. 53-70. https://doi.org/10.1007/978-1-4939-7111-4_6
Bencsik P, Bartekova M, Görbe A, Kiss K, Pálóczi J, Radosinska J et al. MMP activity detection in zymograms. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 53-70. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7111-4_6
Bencsik, Péter ; Bartekova, Monika ; Görbe, A. ; Kiss, Krisztina ; Pálóczi, János ; Radosinska, Jana ; Szűcs, Gergő ; Ferdinandy, Péter. / MMP activity detection in zymograms. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 53-70 (Methods in Molecular Biology).
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