Mitochondrial Ca2+ uptake with and without the formation of high-Ca2+ microdomains

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31 Citations (Scopus)

Abstract

The mitochondrial Ca2+ uniporter has low affinity for Ca2+, therefore it has been assumed that submicromolar Ca2+ signals cannot induce mitochondrial Ca2+ uptake. The close apposition of the plasma membrane or the endoplamic reticulum (ER) to the mitochondria and the limited Ca2+ diffusion in the cytoplasm result in the formation of perimitochondrial high-Ca2+ microdomains (HCMDs) capable of activating mitochondrial Ca2+ uptake. The possibility of mitochondrial Ca2+ uptake at low submicromolar [Ca2+]c has not yet been generally accepted. Earlier we found in permeabilized glomerulosa, luteal and pancreatic β cells that [Ca2+]m increased when [Ca2+]c was raised from 60 nM to less than 200 nM. Here we report data obtained from H295R (adrenocortical) cells transfected with ER-targeted GFP. Cytoplasmic Ca2+ response to angiotensin II was different in mitochondrion-rich and mitochondrion-free domains. The mitochondrial Ca2+ response to angiotensin II correlated with GFP fluorescence indicating the vicinity of ER. When the cells were exposed to K+ (inducing Ca2+ influx), no correlation was found between the mitochondrial Ca2+ signal and the vicinity of the plasma membrane or the ER. The results presented here provide evidence that mitochondrial Ca2+ uptake may occur both with and without the formation of HCMDs within the same cell.

Original languageEnglish
Pages (from-to)527-537
Number of pages11
JournalCell Calcium
Volume40
Issue number5-6
DOIs
Publication statusPublished - Jan 1 2006

Keywords

  • Angiotensin II
  • Ca signal
  • Endoplasmic reticulum
  • Glomerulosa cell
  • H295R cells
  • Microdomains
  • Mitochondria
  • Potassium

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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