Mitochondria respond to Ca2+ already in the submicromolar range

Correlation with redox state

J. G. Pitter, P. Maechler, C. B. Wollheim, A. Spät

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Rapid formation of high-Ca2+ perimitochondrial cytoplasmic microdomains has been shown to evoke mitochondrial Ca2+ signal and activate mitochondrial dehydrogenases, however, the significance of submicromolar cytoplasmic Ca2+ concentrations in the control of mitochondrial metabolism has not been sufficiently elucidated. Here we studied the mitochondrial response to application of Ca2+ at buffered concentrations in permeabilized rat adrenal glomerulosa cells, in an insulin-producing cell line (INS-1/EK-3) and in an osteosarcoma cell line (143BmA-13). Mitochondrial Ca2+ concentration was measured with the fluorescent dye rhod-2 and, using an in situ calibration method, with the mitochondrially targeted luminescent protein mt-aequorin. In both endocrine cell types, mitochondrial Ca2+ concentration increased in response to elevated cytoplasmic Ca2+ concentration (between 60 and 740 nM) and an increase in mitochondrial Ca2+ concentration could be revealed already at a cytoplasmic Ca2+ concentration step from 60-140 nM. Similar responses were observed in the osteosarcoma cell line, although a clearcut response was first observed at 280 nM extramitochondrial Ca2+ only. As examined in glomerulosa cells, graded increases in cytoplasmic Ca2+ concentration were associated with graded increases in the reduction of mitochondrial pyridine nucleotides, consistent with Ca2+-dependent activation of mitochondrial dehydrogenases. Our data indicate that in addition to the recognized role of high-Ca2+ cytoplasmic microdomains, also small Ca2+ signals may influence mitochondrial metabolism.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalCell Calcium
Volume31
Issue number2
DOIs
Publication statusPublished - 2002

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Oxidation-Reduction
Mitochondria
Osteosarcoma
Cell Line
Oxidoreductases
Luminescent Proteins
Aequorin
Zona Glomerulosa
Endocrine Cells
Calibration
Nucleotides
Insulin
pyridine
rhod-2

ASJC Scopus subject areas

  • Cell Biology
  • Endocrinology

Cite this

Mitochondria respond to Ca2+ already in the submicromolar range : Correlation with redox state. / Pitter, J. G.; Maechler, P.; Wollheim, C. B.; Spät, A.

In: Cell Calcium, Vol. 31, No. 2, 2002, p. 97-104.

Research output: Contribution to journalArticle

Pitter, J. G. ; Maechler, P. ; Wollheim, C. B. ; Spät, A. / Mitochondria respond to Ca2+ already in the submicromolar range : Correlation with redox state. In: Cell Calcium. 2002 ; Vol. 31, No. 2. pp. 97-104.
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