Mito-nuclear discordance helps to reveal the phylogeographic patterns of Melitaea ornata (Lepidoptera

Nymphalidae)

János P. Tóth, Z. Varga, Rudi Verovnik, Niklas Wahlberg, Alex Váradi, Judit Bereczki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Periodical changes of glacial and interglacial conditions have influenced the distribution of most living organisms and shape the separation of different genetic lineages significantly. We investigated the phylogeography of a nymphalid butterfly Melitaea ornata. Our main aim was to explore the existence, the origin, and the variability of different genetic lineages based on a multilevel approach. M. ornata and its close relatives (with a focus on M. phoebe) from the Palearctic were analysed based on five gene regions (COI, EF-1a, MDH, RPS5, and wingless) using Bayesian methods to infer the phylogeographic history. The DNA-based analyses have been complemented with species distribution modelling (SDM) and Wolbachia screening. The Bayesian inference analysis showed mito-nuclear discordance in M. ornata, which is split into an eastern and a western clade. Based on mitochondrial DNA, the western clade of M. ornata clusters together with M. phoebe, while the eastern clade is well-separated. In contrast to this, the combined nuDNA-based analysis revealed that M. ornata forms a monophyletic group which is clearly separated from M. phoebe. The timing of divergence analyses suggest that the split between M. ornata and M. phoebe is about 6 million years old based on both the COI and the concatenated nuclear genes. SDM predicted considerably larger area shifts for M. phoebe than for M. ornata. LGM refugia were predicted for both species to the Mediterranean peninsulas in Europe and several Middle-East and Asian localities. The prevalence of Wolbachia infection was 88.9% in M. phoebe and only 7% in M. ornata. Our results clearly indicate a lack of ongoing hybridization between M. phoebe and M. ornata, but argue for an ancient hybridization event in the Apennine Peninsula which strongly influenced the observed split between the two clades of M. ornata.

Original languageEnglish
Pages (from-to)267-281
Number of pages15
JournalBiological Journal of the Linnean Society
Volume121
Issue number2
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Phoebe
Nymphalidae
Lepidoptera
Bayesian analysis
gene
phylogeography
refugium
Wolbachia
butterfly
interglacial
mitochondrial DNA
modeling
Bayesian theory
divergence
hybridization
biogeography
DNA
history
Middle East
distribution

Keywords

  • Hybridization
  • Last Glacial Maximum
  • Species distribution modelling
  • Wolbachia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Mito-nuclear discordance helps to reveal the phylogeographic patterns of Melitaea ornata (Lepidoptera : Nymphalidae). / Tóth, János P.; Varga, Z.; Verovnik, Rudi; Wahlberg, Niklas; Váradi, Alex; Bereczki, Judit.

In: Biological Journal of the Linnean Society, Vol. 121, No. 2, 01.01.2017, p. 267-281.

Research output: Contribution to journalArticle

Tóth, János P. ; Varga, Z. ; Verovnik, Rudi ; Wahlberg, Niklas ; Váradi, Alex ; Bereczki, Judit. / Mito-nuclear discordance helps to reveal the phylogeographic patterns of Melitaea ornata (Lepidoptera : Nymphalidae). In: Biological Journal of the Linnean Society. 2017 ; Vol. 121, No. 2. pp. 267-281.
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