Misregulated RNA Pol II C-terminal domain phosphorylation results in apoptosis

T. Schauer, I. Tombácz, A. Ciurciu, O. Komonyi, I. Boros

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Misregulation of the level of RNA polymerase II carboxyl-terminal domain (CTD) phosphatase, Fcp1, in Drosophila results in high level of caspase-mediated apoptosis. Apoptosis induction by Fcp1 misregulation requires the presence of Drosophila melanogaster (Dm)p53, but occurs without the transcriptional activation of Dmp53 proapoptotic targets rpr, ark, and hid. Overproduction of a transcription activation-defective mutant Dmp53 protein increases, while Dmp53 null background decreases significantly the level of apoptosis in Fcp1-misregulated animals. Generating the apoptotic signal does not require the function of the ATM and Rad3-related kinase (ATR), and no significant level of nucleo-cytoplasmic translocation of Dmp53 is detectable in cells expressing Fcp1 at an abnormal level. Immunostaining of larval salivary gland polytene chromosomes with anti-Dmp53 antibodies indicates Dmp53 localization at several transcriptionally active chromosomal regions in wild-type cells, while in Fcp-misregulated cells the association of Dmp53 with specific chromosomal sites is decreased.

Original languageEnglish
Pages (from-to)909-918
Number of pages10
JournalCellular and Molecular Life Sciences
Volume66
Issue number5
DOIs
Publication statusPublished - Mar 2009

Fingerprint

RNA Polymerase II
Phosphorylation
Apoptosis
Transcriptional Activation
Polytene Chromosomes
Mutant Proteins
Caspases
Salivary Glands
Drosophila melanogaster
Phosphoric Monoester Hydrolases
Drosophila
Anti-Idiotypic Antibodies
Phosphotransferases

Keywords

  • Apoptosis
  • ATM and Rad3-related kinase
  • C-terminal domain
  • P53
  • Polytene chromosome
  • RNA interference
  • RNA polymerase II
  • Transcription blockage

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Misregulated RNA Pol II C-terminal domain phosphorylation results in apoptosis. / Schauer, T.; Tombácz, I.; Ciurciu, A.; Komonyi, O.; Boros, I.

In: Cellular and Molecular Life Sciences, Vol. 66, No. 5, 03.2009, p. 909-918.

Research output: Contribution to journalArticle

Schauer, T. ; Tombácz, I. ; Ciurciu, A. ; Komonyi, O. ; Boros, I. / Misregulated RNA Pol II C-terminal domain phosphorylation results in apoptosis. In: Cellular and Molecular Life Sciences. 2009 ; Vol. 66, No. 5. pp. 909-918.
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