miR-21 is up-regulated in psoriasis and suppresses T cell apoptosis

Florian Meisgen, Ning Xu, Tianling Wei, Peter C. Janson, Susanna Obad, Oliver Broom, N. Nagy, Sakari Kauppinen, L. Kemény, Mona Ståhle, Andor Pivarcsi, Enikö Sonkoly

Research output: Contribution to journalArticle

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Abstract

MicroRNAs are short non-coding RNAs that regulate gene expression. Previously, in a genome-wide screen, we found deregulation of microRNA expression in psoriasis skin. MicroRNA-21 (miR-21) is one of the microRNAs significantly up-regulated in psoriasis skin lesions. To identify the cell type responsible for the increased miR-21 level, we compared expression of miR-21 in epidermal cells and dermal T cells between psoriasis and healthy skin and found elevated levels of miR-21 in psoriasis in both cell types. In cultured T cells, expression of miR-21 increased markedly upon activation. To explore the function of miR-21 in primary human T helper cells, we inhibited miR-21 using a tiny seed-targeting LNA-anti-miR. Specific inhibition of miR-21 increased the apoptosis rate of activated T cells. Our results suggest that miR-21 suppresses apoptosis in activated T cells, and thus, overexpression of miR-21 may contribute to T cell-derived psoriatic skin inflammation.

Original languageEnglish
Pages (from-to)312-314
Number of pages3
JournalExperimental Dermatology
Volume21
Issue number4
DOIs
Publication statusPublished - Apr 2012

Fingerprint

T-cells
MicroRNAs
Psoriasis
Apoptosis
T-Lymphocytes
Skin
Untranslated RNA
Deregulation
Helper-Inducer T-Lymphocytes
Gene expression
Seed
Cultured Cells
Seeds
Genes

Keywords

  • Chronic inflammation
  • Inflammatory skin disease
  • MicroRNA
  • Skin
  • T cells

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Cite this

Meisgen, F., Xu, N., Wei, T., Janson, P. C., Obad, S., Broom, O., ... Sonkoly, E. (2012). miR-21 is up-regulated in psoriasis and suppresses T cell apoptosis. Experimental Dermatology, 21(4), 312-314. https://doi.org/10.1111/j.1600-0625.2012.01462.x

miR-21 is up-regulated in psoriasis and suppresses T cell apoptosis. / Meisgen, Florian; Xu, Ning; Wei, Tianling; Janson, Peter C.; Obad, Susanna; Broom, Oliver; Nagy, N.; Kauppinen, Sakari; Kemény, L.; Ståhle, Mona; Pivarcsi, Andor; Sonkoly, Enikö.

In: Experimental Dermatology, Vol. 21, No. 4, 04.2012, p. 312-314.

Research output: Contribution to journalArticle

Meisgen, F, Xu, N, Wei, T, Janson, PC, Obad, S, Broom, O, Nagy, N, Kauppinen, S, Kemény, L, Ståhle, M, Pivarcsi, A & Sonkoly, E 2012, 'miR-21 is up-regulated in psoriasis and suppresses T cell apoptosis', Experimental Dermatology, vol. 21, no. 4, pp. 312-314. https://doi.org/10.1111/j.1600-0625.2012.01462.x
Meisgen, Florian ; Xu, Ning ; Wei, Tianling ; Janson, Peter C. ; Obad, Susanna ; Broom, Oliver ; Nagy, N. ; Kauppinen, Sakari ; Kemény, L. ; Ståhle, Mona ; Pivarcsi, Andor ; Sonkoly, Enikö. / miR-21 is up-regulated in psoriasis and suppresses T cell apoptosis. In: Experimental Dermatology. 2012 ; Vol. 21, No. 4. pp. 312-314.
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