Minocycline Enhances the Effectiveness of Nociceptin/Orphanin FQ during Neuropathic Pain

Katarzyna Popiolek-Barczyk, Ewelina Rojewska, Agnieszka M. Jurga, Wioletta Makuch, Ferenz Zador, A. Borsodi, Anna Piotrowska, Barbara Przewlocka, Joanna Mika

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Nociceptin/orphanin FQ (N/OFQ) antinociception, which is mediated selectively by the N/OFQ peptide receptor (NOP), was demonstrated in pain models. In this study, we determine the role of activated microglia on the analgesic effects of N/OFQ in a rat model of neuropathic pain induced by chronic constriction injury (CCI) to the sciatic nerve. Repeated 7-day administration of minocycline (30 mg/kg i.p.), a drug that affects microglial activation, significantly reduced pain in CCI-exposed rats and it potentiates the analgesic effects of administered N/OFQ (2.5-5 g i.t.). Minocycline also downregulates the nerve injury-induced upregulation of NOP protein in the dorsal lumbar spinal cord. Our in vitro study showed that minocycline reduced NOP mRNA, but not protein, level in rat primary microglial cell cultures. In [35S]GTPγS binding assays we have shown that minocycline increases the spinal N/OFQ-stimulated NOP signaling. We suggest that the modulation of the N/OFQ system by minocycline is due to the potentiation of its neuronal antinociceptive activity and weakening of the microglial cell activation. This effect is beneficial for pain relief, and these results suggest new targets for the development of drugs that are effective against neuropathic pain.

Original languageEnglish
Article number762930
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

Minocycline
Neuralgia
Peptide Receptors
Rats
Constriction
Analgesics
Spinal Cord
Wounds and Injuries
Chemical activation
nociceptin
Pain
Primary Cell Culture
Microglia
Sciatic Nerve
Cell culture
Chronic Pain
Pharmaceutical Preparations
Assays
Proteins
Up-Regulation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Popiolek-Barczyk, K., Rojewska, E., Jurga, A. M., Makuch, W., Zador, F., Borsodi, A., ... Mika, J. (2014). Minocycline Enhances the Effectiveness of Nociceptin/Orphanin FQ during Neuropathic Pain. BioMed Research International, 2014, [762930]. https://doi.org/10.1155/2014/762930

Minocycline Enhances the Effectiveness of Nociceptin/Orphanin FQ during Neuropathic Pain. / Popiolek-Barczyk, Katarzyna; Rojewska, Ewelina; Jurga, Agnieszka M.; Makuch, Wioletta; Zador, Ferenz; Borsodi, A.; Piotrowska, Anna; Przewlocka, Barbara; Mika, Joanna.

In: BioMed Research International, Vol. 2014, 762930, 2014.

Research output: Contribution to journalArticle

Popiolek-Barczyk, K, Rojewska, E, Jurga, AM, Makuch, W, Zador, F, Borsodi, A, Piotrowska, A, Przewlocka, B & Mika, J 2014, 'Minocycline Enhances the Effectiveness of Nociceptin/Orphanin FQ during Neuropathic Pain', BioMed Research International, vol. 2014, 762930. https://doi.org/10.1155/2014/762930
Popiolek-Barczyk, Katarzyna ; Rojewska, Ewelina ; Jurga, Agnieszka M. ; Makuch, Wioletta ; Zador, Ferenz ; Borsodi, A. ; Piotrowska, Anna ; Przewlocka, Barbara ; Mika, Joanna. / Minocycline Enhances the Effectiveness of Nociceptin/Orphanin FQ during Neuropathic Pain. In: BioMed Research International. 2014 ; Vol. 2014.
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