Minitransfection: a simple, fast technique for transfections

W. Z. Ho, E. Gönczöl, A. Srinivasan, S. D. Douglas, S. A. Plotkin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A fast, simple and inexpensive minitransfection technique, using either a lipofection or a calcium phosphate coprecipitation method to introduce foreign DNA into living cells is presented. This technique is based on the use of 24-well or 96-well tissue culture plates and can be used for both transient and stable transfections. Because it is a microtechnique, only small amounts of DNA, cells and transfection reagents are necessary, and it is easy to handle multiple DNA transfections or cotransfections in different cell lines and in duplicates or triplicates. The technique can be used to study viral gene expression, virus replication and chloramphenicol acetyltransferase (CAT) assay in different cell lines, for example, in the large scale screening and testing of antiviral agents.

Original languageEnglish
Pages (from-to)79-88
Number of pages10
JournalJournal of Virological Methods
Volume32
Issue number1
DOIs
Publication statusPublished - 1991

Fingerprint

Transfection
DNA
Cell Line
Chloramphenicol O-Acetyltransferase
Viral Genes
Virus Replication
Antiviral Agents
Gene Expression
calcium phosphate

Keywords

  • Lipofection
  • Macrotransfection
  • Minitransfection

ASJC Scopus subject areas

  • Virology

Cite this

Minitransfection : a simple, fast technique for transfections. / Ho, W. Z.; Gönczöl, E.; Srinivasan, A.; Douglas, S. D.; Plotkin, S. A.

In: Journal of Virological Methods, Vol. 32, No. 1, 1991, p. 79-88.

Research output: Contribution to journalArticle

Ho, W. Z. ; Gönczöl, E. ; Srinivasan, A. ; Douglas, S. D. ; Plotkin, S. A. / Minitransfection : a simple, fast technique for transfections. In: Journal of Virological Methods. 1991 ; Vol. 32, No. 1. pp. 79-88.
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