Minimal experimental requirements for definition of extracellular vesicles and their functions: A position statement from the International Society for Extracellular Vesicles

Jan L�tvall, Andrew F. Hill, Fred Hochberg, E. Búzás, Dolores Di Vizio, Christopher Gardiner, Yong Song Gho, Igor V. Kurochkin, Suresh Mathivanan, Peter Quesenberry, Susmita Sahoo, Hidetoshi Tahara, Marca H. Wauben, Kenneth W. Witwer, Clotilde Th�ry

Research output: Contribution to journalEditorial

889 Citations (Scopus)

Abstract

Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.

Original languageEnglish
Article number26913
JournalJournal of Extracellular Vesicles
Volume3
Issue number1
DOIs
Publication statusPublished - Jan 1 2014

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Cell-Derived Microparticles
Exosomes
Extracellular Vesicles
Research Personnel
Medicine
Guidelines
Membranes

Keywords

  • Ectosomes
  • Exosomes
  • Extracellular RNA
  • Extracellular vesicles
  • Microparticles
  • Microvesicles

ASJC Scopus subject areas

  • Histology
  • Cell Biology

Cite this

Minimal experimental requirements for definition of extracellular vesicles and their functions : A position statement from the International Society for Extracellular Vesicles. / L�tvall, Jan; Hill, Andrew F.; Hochberg, Fred; Búzás, E.; Vizio, Dolores Di; Gardiner, Christopher; Gho, Yong Song; Kurochkin, Igor V.; Mathivanan, Suresh; Quesenberry, Peter; Sahoo, Susmita; Tahara, Hidetoshi; Wauben, Marca H.; Witwer, Kenneth W.; Th�ry, Clotilde.

In: Journal of Extracellular Vesicles, Vol. 3, No. 1, 26913, 01.01.2014.

Research output: Contribution to journalEditorial

L�tvall, J, Hill, AF, Hochberg, F, Búzás, E, Vizio, DD, Gardiner, C, Gho, YS, Kurochkin, IV, Mathivanan, S, Quesenberry, P, Sahoo, S, Tahara, H, Wauben, MH, Witwer, KW & Th�ry, C 2014, 'Minimal experimental requirements for definition of extracellular vesicles and their functions: A position statement from the International Society for Extracellular Vesicles', Journal of Extracellular Vesicles, vol. 3, no. 1, 26913. https://doi.org/10.3402/jev.v3.26913
L�tvall, Jan ; Hill, Andrew F. ; Hochberg, Fred ; Búzás, E. ; Vizio, Dolores Di ; Gardiner, Christopher ; Gho, Yong Song ; Kurochkin, Igor V. ; Mathivanan, Suresh ; Quesenberry, Peter ; Sahoo, Susmita ; Tahara, Hidetoshi ; Wauben, Marca H. ; Witwer, Kenneth W. ; Th�ry, Clotilde. / Minimal experimental requirements for definition of extracellular vesicles and their functions : A position statement from the International Society for Extracellular Vesicles. In: Journal of Extracellular Vesicles. 2014 ; Vol. 3, No. 1.
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